Expression profiles suggest a role for Pax7 in the establishment of tectal polarity and map refinement

The role for Pax7 in establishing tectal polarity and map refinement was authenticated by gene expression studies in vivo and in vitro. Throughout development (stages E2-E12 were examined) a rostral(low)-caudal(high) and dorsal(high)-ventral(low) Pax7 expression gradient was detected immunohistochem...

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Bibliographic Details
Published in:Experimental brain research 2004-06, Vol.156 (3), p.263-273
Main Authors: Thomas, Meghan, Lazic, Stan, Beazley, Lyn, Ziman, Melanie
Format: Article
Language:English
Subjects:
Animals
Body Patterning - genetics
Cell Communication - genetics
Cell Differentiation - genetics
Cell Polarity - genetics
Cells, Cultured
Chick Embryo
Cues
Ephrin-A2 - genetics
Ephrin-A2 - metabolism
Gene Expression Regulation, Developmental - genetics
Growth Cones - metabolism
Growth Cones - ultrastructure
Homeodomain Proteins - antagonists & inhibitors
Homeodomain Proteins - genetics
Homeodomain Proteins - metabolism
Mice
Neurons - cytology
Neurons - metabolism
Oligonucleotides, Antisense - pharmacology
PAX7 Transcription Factor
Retina - cytology
Retina - embryology
Retina - metabolism
Superior Colliculi - cytology
Superior Colliculi - embryology
Superior Colliculi - metabolism
Transfection
Up-Regulation - genetics
Visual Pathways - cytology
Visual Pathways - embryology
Visual Pathways - metabolism
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Summary:The role for Pax7 in establishing tectal polarity and map refinement was authenticated by gene expression studies in vivo and in vitro. Throughout development (stages E2-E12 were examined) a rostral(low)-caudal(high) and dorsal(high)-ventral(low) Pax7 expression gradient was detected immunohistochemically in the chick optic tectum, indicating a role for Pax7 in establishing tectal polarity. Chick retino-recipient tectal cells positive for Pax7 also co-expressed ephrin-A2, a molecule involved in the establishment and refinement of the retinotopic map. In vitro, PAX7 up-regulated ephrin-A2 when transfected into undifferentiated P19 cells; cells became negative for both Pax7 and ephrin-A2 protein following treatment with anti-sense oligonucleotides. These results suggest that in addition to being involved in the early establishment of tectal polarity, Pax7 plays a later role in retino-tectal map formation and refinement.
ISSN:0014-4819
1432-1106
DOI:10.1007/s00221-003-1775-z