Internalization and trafficking of fluorescent-labeled phospholipids in yeast

Phospholipid reporter molecules, containing a fluorescent group attached to a short, acyl chain, spontaneously insert into the plasma membrane of yeast cells allowing retrograde trafficking to intracellular organelles as well as their metabolic fates to be monitored. This approach provides the frame...

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Bibliographic Details
Published in:Seminars in cell & developmental biology 2002-06, Vol.13 (3), p.179-184
Main Author: Nichols, J.Wylie
Format: Article
Language:English
Subjects:
Cell Membrane - metabolism
flip–flop
Fluorescent Dyes - pharmacology
Models, Biological
NBD
Phospholipids - metabolism
plasma membrane
Protein Transport
Saccharomyces cerevisiae
Saccharomyces cerevisiae - metabolism
Temperature
Time Factors
vacuole
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Summary:Phospholipid reporter molecules, containing a fluorescent group attached to a short, acyl chain, spontaneously insert into the plasma membrane of yeast cells allowing retrograde trafficking to intracellular organelles as well as their metabolic fates to be monitored. This approach provides the framework for determining the dependence of particular phospholipid trafficking and metabolic steps on a wide range of genes known to be required for related membrane transport functions as well as for developing genetic screens to identify novel genes required for these processes. This review presents an overview of insights gained into phospholipid trafficking and metabolism using this approach.
ISSN:1084-9521
1096-3634
DOI:10.1016/S1084-9521(02)00046-0