Estrogen receptors in atherosclerotic human aorta: inhibition of human vascular smooth muscle cell proliferation by estrogens

Estrogen has been postulated to exert direct anti-atherogenic effects via binding to estrogen receptors (ERs) in vascular smooth muscle cells (VSMCs). Therefore, we believe it is important to examine the status of ER expression in the human cardiovascular system and its disorders. In this study, we...

Full description

Saved in:
Bibliographic Details
Published in:Molecular and cellular endocrinology 2004-04, Vol.219 (1), p.17-26
Main Authors: Nakamura, Yasuhiro, Suzuki, Takashi, Miki, Yasuhiro, Tazawa, Chika, Senzaki, Kumiko, Moriya, Takuya, Saito, Haruo, Ishibashi, Tadashi, Takahashi, Shoki, Yamada, Shogo, Sasano, Hironobu
Format: Article
Language:English
Subjects:
Actins - chemistry
Aorta - chemistry
Aorta - metabolism
Aorta - pathology
Arteriosclerosis - genetics
Arteriosclerosis - metabolism
Atherosclerosis
Cell Proliferation - drug effects
Estrogen receptor
Estrogen Receptor alpha - analysis
Estrogen Receptor alpha - genetics
Estrogen Receptor beta - analysis
Estrogen Receptor beta - genetics
Estrogens - pharmacology
Female
Gene Expression Regulation
Humans
Male
Middle Aged
Muscle, Smooth, Vascular - drug effects
Proliferating Cell Nuclear Antigen - genetics
Receptors, Estrogen - analysis
Receptors, Estrogen - genetics
Receptors, Estrogen - metabolism
Vascular smooth muscle cells
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Estrogen has been postulated to exert direct anti-atherogenic effects via binding to estrogen receptors (ERs) in vascular smooth muscle cells (VSMCs). Therefore, we believe it is important to examine the status of ER expression in the human cardiovascular system and its disorders. In this study, we first evaluated the relative abundance of messenger RNA (mRNA) of both ER subtypes (ERα and ERβ) in the human aorta using reverse transcription followed by quantitative polymerase chain reaction (RT-qPCR). We then examined the immunolocalization of both ERs in VSMCs of human atherosclerotic lesions. In order to examine which ER subtype was associated with the anti-atherogenic effects of estrogen, we examined the effects of estrogen in two VSMC cell lines, one positive only for ERα and the other positive only for ERβ. The relative abundance of mRNAs for both ERs was higher in female aorta with a mild degree of atherosclerosis than in female aorta with a severe degree of atherosclerosis ( P
ISSN:0303-7207
1872-8057
DOI:10.1016/j.mce.2004.02.013