CpG-containing oligodeoxynucleotides augment and switch the immune responses of cattle to bovine herpesvirus-1 glycoprotein D

The adjuvanticity of a synthetic oligodeoxynucleotide containing unmethylated CpG motifs (CpG ODN) was determined in cattle. Calves were immunized with a truncated secreted version of glycoprotein D (tgD) of bovine herpes virus-1 (BHV-1) formulated with alum, CpG ODN, or a combination of both. BHV-1...

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Bibliographic Details
Published in:Vaccine 2002-07, Vol.20 (23), p.3014-3022
Main Authors: Rankin, R., Pontarollo, R., Gomis, S., Karvonen, B., Willson, P., Loehr, B.I., Godson, D.L., Babiuk, L.A., Hecker, R., van Drunen Littel-van den Hurk, S.
Format: Article
Language:English
Subjects:
Adjuvant
Adjuvants, Immunologic - administration & dosage
Adjuvants, Immunologic - adverse effects
Alum Compounds - administration & dosage
Animals
Antibodies, Viral - blood
Antigens, Viral
Base Sequence
Bovine herpesvirus 1
Cattle
Cattle Diseases - immunology
Cattle Diseases - prevention & control
CpG-oligodeoxynucleotides
Herpesviridae Infections - immunology
Herpesviridae Infections - prevention & control
Herpesviridae Infections - veterinary
Herpesvirus 1, Bovine - immunology
Immunity, Cellular
Oligodeoxyribonucleotides - administration & dosage
Oligodeoxyribonucleotides - adverse effects
Oligodeoxyribonucleotides - genetics
Skin - drug effects
Skin - immunology
Skin - pathology
Viral Proteins - immunology
Viral Vaccines - administration & dosage
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Summary:The adjuvanticity of a synthetic oligodeoxynucleotide containing unmethylated CpG motifs (CpG ODN) was determined in cattle. Calves were immunized with a truncated secreted version of glycoprotein D (tgD) of bovine herpes virus-1 (BHV-1) formulated with alum, CpG ODN, or a combination of both. BHV-1 tgD formulated with CpG ODN or with alum and CpG ODN induced a stronger and more balanced immune response than tgD in alum. This level of immunity was of sufficient magnitude to minimize weight loss and significantly reduce the duration of virus shedding after intranasal viral challenge. Local tissue reactions generated by CpG ODN were very mild and transient, whereas reactions induced by alum or a combination of CpG ODN and alum were moderate in severity and duration. These data demonstrate that CpG ODN causes minimal injection site reactions and yet acts as an effective adjuvant in cattle.
ISSN:0264-410X
1873-2518
DOI:10.1016/S0264-410X(02)00216-5