Genotyping on a Complementary Metal Oxide Semiconductor Silicon Polymerase Chain Reaction Chip with Integrated DNA Microarray

• Published in: Analytical chemistry (Washington) 2002-07, Vol.74 (13), p.3168-3173
• Main Authors: Trau, Dieter, Lee, Thomas M. H, Lao, Alex I. K, Lenigk, Ralf, Hsing, I-Ming, Ip, Nancy Y, Carles, Maria C, Sucher, Nikolaus J
• Format: Article
• Language: English
• Subjects: Biological and medical sciences; CMOS integrated circuits; Deoxyribonucleic acid; DNA; Fundamental and applied biological sciences. Psychology; Genetic engineering; Genetics; Genotype; Microcomputers; Microscopy, Confocal; Molecular and cellular biology; Molecular genetics; Oligonucleotide Array Sequence Analysis - instrumentation; Oligonucleotide Probes; Reverse Transcriptase Polymerase Chain Reaction - instrumentation; Spectrometry, Fluorescence; Temperature control; Transcription. Transcription factor. Splicing. Rna processing
• ISSN: 0003-2700; 1520-6882
• DOI: 10.1021/ac020053u

A novel method for the fast identification of genetic material utilizing a micro-DNA amplification and analysis device (μ-DAAD) consisting of multiple PCR microreactors with integrated DNA microarrays was developed. The device was fabricated in Si-technology and used for the genotyping of Chinese medicinal plants on the basis of differences in the noncoding region of the 5S-rRNA gene. Successful amplification of the genetic material and the consecutive analysis of the fluorescent-labeled amplicons in the μ-DAAD by the integrated oligonucleotide probes were demonstrated. Parallel analysis was performed by loading the four PCR reactors of the μ-DAAD with different samples of 3-μL volume. Temperature sensors and heating elements of the μ-DAAD enable precise temperature control and fast cycling, allowing the rapid completion of a combined amplification and analysis (hybridization) experiment.