TcZFP1: a CCCH zinc finger protein of Trypanosoma cruzi that binds poly-C oligoribonucleotides in vitro

We have identified two zinc finger proteins of Trypanosoma cruzi, the protozoan parasite that causes Chagas disease in humans. These proteins, named tcZFP1 and tcZFP2, share the unusual zinc finger motif (CCCH) found in a diverse range of RNA-binding proteins involved in various aspects of the contr...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2004-06, Vol.319 (1), p.169-177
Main Authors: Mörking, Patrı́cia A, Dallagiovanna, Bruno M, Foti, Leonardo, Garat, Beatriz, Picchi, Gisele F.A, Umaki, Adriana C.S, Probst, Christian M, Krieger, Marco A, Goldenberg, Samuel, Fragoso, Stenio P
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Blotting, Northern
CCCH zinc finger protein
Cloning, Molecular
DNA-Binding Proteins - chemistry
DNA-Binding Proteins - metabolism
Dose-Response Relationship, Drug
EMSA
Green Fluorescent Proteins
Kinetics
Luminescent Proteins - chemistry
Metacyclogenesis
Molecular Sequence Data
Oligoribonucleotides - chemistry
Polyribosomes - chemistry
Protein Binding
Protein Structure, Tertiary
Protozoan Proteins - chemistry
Protozoan Proteins - metabolism
Recombinant Fusion Proteins - chemistry
Recombinant Proteins - chemistry
RNA-Binding Proteins - chemistry
Sequence Analysis, DNA
Sequence Homology, Amino Acid
Time Factors
Trypanosoma cruzi
Trypanosoma cruzi - metabolism
Zinc Fingers
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Summary:We have identified two zinc finger proteins of Trypanosoma cruzi, the protozoan parasite that causes Chagas disease in humans. These proteins, named tcZFP1 and tcZFP2, share the unusual zinc finger motif (CCCH) found in a diverse range of RNA-binding proteins involved in various aspects of the control of cell homeostasis and differentiation. We report here the functional expression of a recombinant tcZFP1, and the relative affinity and stability of the specific complexes formed between the protein and synthetic oligoribonucleotides containing C-rich sequences.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2004.04.162