Efficient Targeting of Gastric Cancer Cells Using Radiolabeled Anti-carcinoembryonic Antigen-specific T84.66 Fragments in Experimental Radioimmunoguided Surgery

Background: Radioimmunoguided surgery (RIGS) appears as an efficient tool for accurate tumor detection up to the level of micrometastases by detecting radiolabeled antibody-bound tumor cells during operation. Anti-CEA-specific T84.66 fragments were examined as to whether they efficiently detected ga...

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Bibliographic Details
Published in:Anticancer research 2004-03, Vol.24 (2B), p.663-670
Main Authors: KIM, Jin C, HONG, Hyun K, RYU, Jin S, KIM, Jung S, KIM, Hee C, ROH, Seon A, KOO, Kum H, KA, In H, NAMGUNG, Hwan, LEE, Kang H, RYU, Jang H, YU, Chang S, OH, Seung J
Format: Article
Language:English
Subjects:
Animals
Autoradiography
Biological and medical sciences
Carcinoembryonic Antigen - immunology
Carcinoembryonic Antigen - metabolism
Female
Gastroenterology. Liver. Pancreas. Abdomen
Humans
Immunoconjugates - immunology
Immunoconjugates - therapeutic use
Immunoglobulin Fragments - immunology
Immunoglobulin Fragments - metabolism
Iodine Radioisotopes
Medical sciences
Mice
Mice, Nude
Radionuclide Imaging
Stomach Neoplasms - diagnostic imaging
Stomach Neoplasms - immunology
Stomach Neoplasms - metabolism
Stomach Neoplasms - surgery
Stomach. Duodenum. Small intestine. Colon. Rectum. Anus
Tumors
Xenograft Model Antitumor Assays
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Summary:Background: Radioimmunoguided surgery (RIGS) appears as an efficient tool for accurate tumor detection up to the level of micrometastases by detecting radiolabeled antibody-bound tumor cells during operation. Anti-CEA-specific T84.66 fragments were examined as to whether they efficiently detected gastric cancer cells in experimental RIGS. T84.66, anti-CEA-specific antibody, has widely been used as an immune carrier in the preclinical and clinical trials of radioimmunotherapy and radioimmunoscintiscan. Materials and Methods: Fifty-one tumors from two human gastric carcinoma cell lines with profuse (MKN45) and low (RF48) CEA expression were successfully implanted subcutaneously in the backs of 32 nude mice. Tumors were localized after 125 I-labeled T84.66 F(ab') 2 and Fab' injection. Results: The radioactivity of F(ab') 2 -pretreated mice was greater than that of Fab'-pretreated in all organs and tumors (p
ISSN:0250-7005
1791-7530