EVALUATION AND COMPARISON OF AN INDIRECT FLUORESCENT ANTIBODY TEST FOR DETECTION OF ANTIBODIES TO SARCOCYSTIS NEURONA, USING SERUM AND CEREBROSPINAL FLUID OF NATURALLY AND EXPERIMENTALLY INFECTED, AND VACCINATED HORSES

The objectives of this study were to evaluate the accuracy of the indirect fluorescent antibody test (IFAT) using serum and cerebrospinal fluid (CSF) of horses naturally and experimentally infected with Sarcocystis neurona, to assess the correlation between serum and CSF titers, and to determine the...

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Bibliographic Details
Published in:The Journal of parasitology 2004-04, Vol.90 (2), p.379-386
Main Authors: Duarte, Paulo C, Daft, Barbara M, Conrad, Patricia A, Packham, Andrea E, Saville, William J, MacKay, Robert J, Barr, Bradd C, David Wilson, W, Ng, Terry, Reed, Stephen M, Gardner, Ian A
Format: Article
Language:English
Subjects:
Animals
Antibodies
Antibodies, Protozoan - blood
Antibodies, Protozoan - cerebrospinal fluid
antibody detection
antibody formation
Biological and medical sciences
blood serum
Central nervous system
cerebrospinal fluid
disease diagnosis
Encephalomyelitis - diagnosis
Encephalomyelitis - parasitology
Encephalomyelitis - veterinary
equine protozoal myeloencephalitis
experimental infection
Experimentation
Female
fluorescent antibody technique
Fluorescent Antibody Technique, Indirect - veterinary
Fundamental and applied biological sciences. Psychology
General aspects
General aspects and techniques. Study of several systematic groups. Models
horse diseases
Horse Diseases - blood
Horse Diseases - cerebrospinal fluid
Horse Diseases - immunology
Horses
infection
Infections
Invertebrates
Likelihood Functions
Male
natural infection
Nervous system diseases
Parasites
Parasitology
Protozoan Vaccines - immunology
Reproducibility of Results
ROC Curve
Sarcocystis - immunology
Sarcocystis neurona
Sarcocystosis - blood
Sarcocystosis - cerebrospinal fluid
Sarcocystosis - immunology
Sarcocystosis - veterinary
Sensitivity and Specificity
serodiagnosis
THERAPEUTICS-DIAGNOSTICS
Vaccination
Vaccination - veterinary
Veterinary medicine
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Summary:The objectives of this study were to evaluate the accuracy of the indirect fluorescent antibody test (IFAT) using serum and cerebrospinal fluid (CSF) of horses naturally and experimentally infected with Sarcocystis neurona, to assess the correlation between serum and CSF titers, and to determine the effect of S. neurona vaccination on the diagnosis of infection. Using receiver-operating characteristic analysis, the areas under the curve for the IFAT were 0.97 (serum) and 0.99 (CSF). Sensitivity and specificity were 83.3 and 96.9% (serum, cutoff 80) and 100 and 99% (CSF, cutoff 5), respectively. Titer-specific likelihood ratios (LRs) ranged from 0.03 to 187.8 for titers between
ISSN:0022-3395
1937-2345
DOI:10.1645/GE-3263