The moleskin gene product is essential for Caudal-mediated constitutive antifungal Drosomycin gene expression in Drosophila epithelia

The homeobox gene, Caudal, encodes the DNA‐binding nuclear transcription factor that plays a crucial role during development and innate immune response. The Drosophila homologue of importin‐7 (DIM‐7), encoded by moleskin, was identified as a Caudal‐interacting molecule during yeast two‐hybrid screen...

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Bibliographic Details
Published in:Insect molecular biology 2004-06, Vol.13 (3), p.323-327
Main Authors: Han, S.-H., Ryu, J.-H., Oh, C.-T., Nam, K.-B., Nam, H.-J., Jang, I.-H., Brey, P. T., Lee, W.-J.
Format: Article
Language:English
Subjects:
Animals
Antifungal Agents - metabolism
Caudal
Drosomycin
Drosophila
Drosophila - genetics
Drosophila - metabolism
Drosophila importin-7
Drosophila Proteins - metabolism
Gene Expression
Genes, Reporter
Glutathione Transferase - metabolism
Homeodomain Proteins - metabolism
Immunity, Innate - genetics
Karyopherins - genetics
Karyopherins - metabolism
Luciferases - metabolism
Microscopy, Confocal
nuclear localization
RNA Interference
Transcription Factors
Two-Hybrid System Techniques
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Summary:The homeobox gene, Caudal, encodes the DNA‐binding nuclear transcription factor that plays a crucial role during development and innate immune response. The Drosophila homologue of importin‐7 (DIM‐7), encoded by moleskin, was identified as a Caudal‐interacting molecule during yeast two‐hybrid screening. Both mutation of the minimal region of Caudal responsible for moleskin binding and RNA interference (RNAi) of moleskin dramatically inhibited the Caudal nuclear localization. Furthermore, Caudal‐mediated constitutive expression of antifungal Drosomycin gene was severely affected in the moleskin‐RNAi flies, showing a local Drosomycin expression pattern indistinguishable from that of the Caudal‐RNAi flies. These in vivo data suggest that DIM‐7 mediates Caudal nuclear localization, which is important for the proper Caudal function necessary for regulating innate immune genes in Drosophila.
ISSN:0962-1075
1365-2583
DOI:10.1111/j.0962-1075.2004.00491.x