Resonance Raman Quantification of Nutritionally Important Carotenoids in Fruits, Vegetables, and Their Juices in Comparison to High-Pressure Liquid Chromatography Analysis

A rapid nondestructive estimation of carotenoid levels in intact fruits and vegetables and their juices could have great value when selecting nutritionally valuable crops for further propagation and commercial use. Carotenoid levels of a variety of agricultural products and juices were measured usin...

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Bibliographic Details
Published in:Journal of agricultural and food chemistry 2004-06, Vol.52 (11), p.3281-3285
Main Authors: Bhosale, Prakash, Ermakov, Igor V, Ermakova, Maia R, Gellermann, Werner, Bernstein, Paul S
Format: Article
Language:English
Subjects:
Beverages - analysis
Biological and medical sciences
Carotenoids - analysis
Chromatography, High Pressure Liquid
Food industries
Fruit - chemistry
Fruit and vegetable industries
Fundamental and applied biological sciences. Psychology
Spectrum Analysis, Raman - methods
Vegetables - chemistry
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Summary:A rapid nondestructive estimation of carotenoid levels in intact fruits and vegetables and their juices could have great value when selecting nutritionally valuable crops for further propagation and commercial use. Carotenoid levels of a variety of agricultural products and juices were measured using resonance Raman spectroscopy and compared to levels determined by extraction and high-pressure liquid chromatography. A strong correlation was observed between the two methods when evaluating juices and when comparing different strains of intact tomatoes at the same stage of ripening. Keywords: β-Carotene; lycopene; lutein; zeaxanthin; Raman spectroscopy; HPLC
ISSN:0021-8561
1520-5118
DOI:10.1021/jf035345q