Addition of p-Azido-l-phenylalanine to the Genetic Code of Escherichia coli

We report the selection of a new orthogonal aminoacyl tRNA synthetase/tRNA pair for the in vivo incorporation of a photocrosslinker, p-azido-l-phenylalanine, into proteins in response to the amber codon, TAG. The amino acid is incorporated in good yield with high fidelity and can be used to crosslin...

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Bibliographic Details
Published in:Journal of the American Chemical Society 2002-08, Vol.124 (31), p.9026-9027
Main Authors: CHIN, Jason W., SANTORO, Stephen W., MARTIN, Andrew B., KING, David S., WANG, Lei, SCHULTZ, Peter G.
Format: Article
Language:English
Subjects:
Azides - metabolism
Bacteriology
Biological and medical sciences
Chloramphenicol O-Acetyltransferase - metabolism
Cross-Linking Reagents
DNA, Bacterial - genetics
DNA, Bacterial - metabolism
Escherichia coli - genetics
Escherichia coli - metabolism
Fundamental and applied biological sciences. Psychology
Methanococcus - enzymology
Microbiology
Miscellaneous
Myoglobin - biosynthesis
Phenylalanine - analogs & derivatives
Phenylalanine - metabolism
Spectrometry, Mass, Electrospray Ionization
Tyrosine-tRNA Ligase - metabolism
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Summary:We report the selection of a new orthogonal aminoacyl tRNA synthetase/tRNA pair for the in vivo incorporation of a photocrosslinker, p-azido-l-phenylalanine, into proteins in response to the amber codon, TAG. The amino acid is incorporated in good yield with high fidelity and can be used to crosslink interacting proteins.
ISSN:0002-7863
1520-5126
DOI:10.1021/ja027007w