Gene Expression in the Cyanobacterium Anabaena sp. PCC7120 under Desiccation

• Published in: Microbial ecology 2004-02, Vol.47 (2), p.164-174
• Main Authors: Katoh, H., Asthana, R. K., Ohmori, M.
• Format: Article
• Language: English
• Subjects: Acclimatization; Anabaena; Anabaena - genetics; Anabaena - metabolism; Anabaena - physiology; Bacteriology; Biological and medical sciences; Biological Transport, Active - physiology; Cyanobacteria; Cyanophyta; Dehydration - metabolism; Deoxyribonucleic acid; Desiccation; DNA; DNA Primers; DNA-Directed RNA Polymerases - metabolism; Down regulation; Fluorescence; Fundamental and applied biological sciences. Psychology; Gene expression; Gene Expression Regulation; Genes; Genomes; Heat-Shock Proteins - metabolism; Low temperature; Marine; Microbiology; Miscellaneous; Nitrogen fixation; Oligonucleotide Array Sequence Analysis; Photosynthesis; Potassium - metabolism; Reverse Transcriptase Polymerase Chain Reaction; RNA; RNA, Messenger - metabolism; Up regulation; Water-Electrolyte Balance - physiology
• ISSN: 0095-3628; 1432-184X
• DOI: 10.1007/s00248-003-1043-6

The N₂-fixing cyanobacterium Anabaena sp. PCC7120 showed an inherent capacity for desiccation tolerance. A DNA microarray covering almost the entire genome of Anabaena was used to determine the genome-wide gene expression under desiccation. RNA was extracted from cells at intervals starting from early to late desiccation. The pattern of gene expression in DNA fragments was categorized into seven types, which include four types of up-regulated and three types of down-regulated fragments. Validation of the data was carried out by RT-PCR on selected up-regulated DNA fragments and was consistent with the changes in mRNA levels. Our conclusions regarding desiccation tolerance for Anabaena sp. PCC7120 are as follows: (i) Genes for osmoprotectant metabolisms and the K⁺ transporting system are upregulated from early to mid-desiccation; (ii) genes induced by osmotic, salt, and low-temperature stress are up-regulated under desiccation; (iii) genes for heat shock proteins are up-regulated after mid-desiccation; (iv) genes for photosynthesis and the nitrogen-transporting system are down-regulated during early desiccation; and (v) genes for RNA polymerase and ribosomal protein are down-regulated between the early and the middle phase of desiccation. Profiles of gene expression are discussed in relation to desiccation acclimation.