Evaluation of a new generation of culture bottle using an automated bacterial culture system for detecting nine common contaminating organisms found in platelet components

• Published in: Transfusion (Philadelphia, Pa.) Pa.), 2002-06, Vol.42 (6), p.774-779
• Main Authors: Brecher, M.E., Heath, D.G., Hay, S.N., Rothenberg, S.J., Stutzman, L.C.
• Format: Article
• Language: English
• Subjects: Aerobiosis; Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy; Automation; Bacillus cereus - growth & development; Bacillus cereus - isolation & purification; Bacteria - growth & development; Bacteria - isolation & purification; Bacteriological Techniques - instrumentation; Biological and medical sciences; Blood Platelets - microbiology; Blood Preservation; Blood. Blood and plasma substitutes. Blood products. Blood cells. Blood typing. Plasmapheresis. Apheresis; Enterobacter cloacae - growth & development; Enterobacter cloacae - isolation & purification; Equipment Design; Escherichia coli - growth & development; Escherichia coli - isolation & purification; Hematology; Humans; Investigative techniques, diagnostic techniques (general aspects); Klebsiella - growth & development; Klebsiella - isolation & purification; Medical sciences; Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques; Platelet Transfusion; Propionibacterium acnes - growth & development; Propionibacterium acnes - isolation & purification; Serratia marcescens - growth & development; Serratia marcescens - isolation & purification; Staphylococcus aureus - growth & development; Staphylococcus aureus - isolation & purification; Streptococcus - growth & development; Streptococcus - isolation & purification; Transfusions. Complications. Transfusion reactions. Cell and gene therapy
• ISSN: 0041-1132; 1537-2995
• DOI: 10.1046/j.1537-2995.2002.00122.x

BACKGROUND : An automated bacterial culture system (BacT/ALERT 3D, bioMérieux) has been previously validated with a variety of bacteria in platelets. The recovery of bacteria in platelets using a new generation of culture bottles that do not require venting and that use a liquid emulsion sensor was studied. STUDY DESIGN AND METHODS : Bacillus cereus, Enterobacter cloacae, Escherichia coli, Klebsiella oxytoca, Staphylococcus aureus, Staphylococcus epidermidis, Serratia marcescens, Streptococcus viridans, and Propionibacterium acnes isolates were inoculated into Day 2 platelets to concentrations of 10 and 100 CFU per mL. Samples were then studied with current and new aerobic, anaerobic, and pediatric bottles. RESULTS : All organisms, except P. acnes, were detected in a mean time of 9.2 to 20.4 (10 CFU/mL) or 8.7 to 18.6 (100 CFU/mL) hours. P. acnes was detected in a mean time of 69.2 (10 CFU/mL) or 66.0 (100 CFU/mL) hours. The 10‐fold increase in inoculum was associated with a mean 9.2 percent difference in detection time. The aerobic, anaerobic, and pediatric bottles had a mean difference in detection time (hours) between the current and new bottles of 0.10 (p = 0.61), 0.4 (p = 0.38), and 1.0 (p < 0.001), respectively. CONCLUSION : No difference in detection time between the current and new aerobic and anaerobic bottles was demonstrated. The new pediatric bottles had a small but significant delay in detection.