Plant regeneration from hairy-root cultures transformed by infection with Agrobacterium rhizogenes in Catharanthus roseus

Hypocotyl explants of Catharanthus roseus produced hairy roots when cultured on Murashige and Skoog (MS) basal medium after infection by Agrobacterium rhizogenes. Explants gave rise to adventitious shoots at a frequency of up to 80% when cultured on MS medium supplemented with 31.1 micromolar 6-benz...

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Bibliographic Details
Published in:Plant cell reports 2004-06, Vol.22 (11), p.828-831
Main Authors: Choi, P.S, Kim, Y.D, Choi, K.M, Chung, H.J, Choi, D.W, Liu, J.R
Format: Article
Language:English
Subjects:
Adenine - analogs & derivatives
Adenine - pharmacology
adventitious shoots
Agrobacterium rhizogenes
Benzyl Compounds
benzyladenine
callus culture
Catharanthus - genetics
Catharanthus roseus
Cultivars
culture media
Culture Media - pharmacology
Flowers
genetic transformation
in vitro regeneration
Kinetin
micropropagation
molecular sequence data
naphthaleneacetic acid
Naphthaleneacetic Acids - pharmacology
nucleotide sequences
ornamental plants
plant regeneration
Plant Roots - drug effects
Plant Roots - genetics
Plant Roots - growth & development
Plants, Genetically Modified
Purines
Regeneration
Rhizobium - genetics
Rhizobium rhizogenes
root hairs
Roots
Transformation, Genetic
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Summary:Hypocotyl explants of Catharanthus roseus produced hairy roots when cultured on Murashige and Skoog (MS) basal medium after infection by Agrobacterium rhizogenes. Explants gave rise to adventitious shoots at a frequency of up to 80% when cultured on MS medium supplemented with 31.1 micromolar 6-benzyladenine and 5.4 micromolar alpha-naphthaleneacetic acid. There was a significant difference in the frequency of adventitious shoot formation for each hairy-root line derived from a different cultivar. Plants derived from hairy roots exhibited prolific rooting and had shortened internodes. Approximately half of the plants had wrinkled leaves and an abundant root mass with extensive lateral branching, but otherwise appeared morphologically normal. Plants with hairy roots that were derived from the cultivar Cooler Apricot developed flowers with petals that were white in the proximal region, whereas the wild-type flower petals are red. PCR and Southern blot analyses revealed that plants derived from hairy roots retained the Ri TL-DNA.
ISSN:0721-7714
1432-203X
DOI:10.1007/s00299-004-0765-3