Partial purification and characterization of Gigantocotyle explanatum somatic antigens

Soluble extracts of Gigantocotyle explanatum, isolated from the liver of buffalo Bubalus bubalis were fractionated on Sephadex G-200 columns. Nine major fractions referred to as F1, F2, F3, F4, F5, F6, F7, F8 and F9 were separated. Each fraction was tested by ELISA for antigenicity using sera from G...

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Bibliographic Details
Published in:Journal of helminthology 2004-06, Vol.78 (2), p.95-99
Main Authors: Ahmad, G., Saifullah, M.K., Nizami, W.A.
Format: Article
Language:English
Subjects:
Animals
Antigens, Helminth - chemistry
Antigens, Helminth - isolation & purification
Biological and medical sciences
Blotting, Western - methods
Buffaloes - parasitology
Electrophoresis, Polyacrylamide Gel - methods
Enzyme-Linked Immunosorbent Assay - methods
Fundamental and applied biological sciences. Psychology
Host parasite relation
pathogenicity
Invertebrates
Liver - parasitology
Molecular Weight
Nemathelminthia. Plathelmintha
Paramphistomatidae - immunology
Trematode Infections - immunology
Trematode Infections - veterinary
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Summary:Soluble extracts of Gigantocotyle explanatum, isolated from the liver of buffalo Bubalus bubalis were fractionated on Sephadex G-200 columns. Nine major fractions referred to as F1, F2, F3, F4, F5, F6, F7, F8 and F9 were separated. Each fraction was tested by ELISA for antigenicity using sera from G. explanatum-infected field buffaloes. Fractions F1 and F2 were highly antigenic, F3, F4, F6 and F7 were moderately antigenic and F5, F8 and F9 were poorly antigenic. Analyses by SDS–PAGE revealed that each fraction comprised several polypeptide(s) in the molecular weight range of 205 kDa. Results of Western blotting indicated that not all polypeptides which appeared in the SDS–PAGE were antigenic. The antigenic molecules of each fraction were mostly in the low molecular weight range of 94 kDa with the polypeptides in the range of >14, 14, 18, 21–25 and 34–36 kDa.
ISSN:0022-149X
1475-2697
DOI:10.1079/JOH2003228