Capillary electrophoresis monitors changes in the electrophoretic behavior of mitochondrial preparations

The presence of electrical charges on the surface of an organelle is the source of the organelle’s electrophoretic mobility. Recently, we reported that capillary electrophoresis with laser-induced fluorescence detection (CE-LIF) can be used to determine the electrophoretic mobility of individual mit...

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Bibliographic Details
Published in:Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2004-07, Vol.806 (2), p.151-159
Main Authors: Fuller, Kathryn M, Arriaga, Edgar A
Format: Article
Language:English
Subjects:
Analysis
Analytical, structural and metabolic biochemistry
Animals
Biological and medical sciences
Electrophoresis, Capillary - methods
Electrophoretic mobility
Fluorescence
Freezing
Fundamental and applied biological sciences. Psychology
General pharmacology
Medical sciences
Mice
Mitochondria, Liver - physiology
Mitochondrial preparations
Pharmacology. Drug treatments
Rats
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Summary:The presence of electrical charges on the surface of an organelle is the source of the organelle’s electrophoretic mobility. Recently, we reported that capillary electrophoresis with laser-induced fluorescence detection (CE-LIF) can be used to determine the electrophoretic mobility of individual mitochondria. Here, we describe the use of CE-LIF to monitor changes in the electrophoretic mobility distributions of: (i) mitochondria isolated from cultured NS-1 mouse hybridoma cells disrupted by nitrogen cavitation or mechanical homogenization; (ii) mitochondria isolated from rat liver and purified by gradient centrifugation before and after being frozen in liquid nitrogen; and (iii) mitochondria chemically transformed into mitoplasts. These results indicate that the organelle electrophoretic mobility observed by researchers is affected by preparation procedures and that CE-LIF is a complementary technique for monitoring the quality of mitochondrial preparations.
ISSN:1570-0232
1873-376X
DOI:10.1016/j.jchromb.2004.03.050