Amelogenin is a Cell Adhesion Protein

Amelogenin, the major protein component of tooth enamel, is shown to be a cell adhesion protein. Since it had been shown that an amelogenin-containing preparation, Emdogain®, possessed cell-adhesive activity, we tested the hypothesis that amelogenin was responsible for cell-adhesive activity. Recomb...

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Bibliographic Details
Published in:Journal of dental research 2002-07, Vol.81 (7), p.497-500
Main Authors: Hoang, A.M., Klebe, R.J., Steffensen, B., Ryu, O.H., Simmer, J.P., Cochran, D.L.
Format: Article
Language:English
Subjects:
Amelogenin
Biocompatible Materials - chemistry
Calcium - pharmacology
Cell Adhesion - drug effects
Cell Adhesion Molecules - metabolism
Cell Adhesion Molecules - pharmacology
Cell Adhesion Molecules - physiology
Cell Culture Techniques
Collagen Type I - metabolism
Dental Enamel Proteins - metabolism
Dental Enamel Proteins - pharmacology
Dental Enamel Proteins - physiology
Dentistry
Durapatite - chemistry
Extracellular Matrix Proteins - metabolism
Heparin - metabolism
Humans
Magnesium - pharmacology
Osteosarcoma - metabolism
Osteosarcoma - pathology
Periodontal Ligament - cytology
Periodontal Ligament - metabolism
Recombinant Proteins
Surface Properties
Tumor Cells, Cultured
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Summary:Amelogenin, the major protein component of tooth enamel, is shown to be a cell adhesion protein. Since it had been shown that an amelogenin-containing preparation, Emdogain®, possessed cell-adhesive activity, we tested the hypothesis that amelogenin was responsible for cell-adhesive activity. Recombinant amelogenin was found to promote adhesion at less than 15 μg/60-mm plate and requires divalent cations for activity. While we found that amelogenin does not bind to collagen or heparin under physiological conditions, it was demonstrated previously that amelogenin does bind to hydroxyapatite. The cell-adhesive activity of amelogenin may play a role in development and may provide a partial explanation for the therapeutic effects of Emdogain® in periodontal regeneration.
ISSN:0022-0345
1544-0591
DOI:10.1177/154405910208100713