A study into the anti-microbial properties of an amino functionalised polymer using multi-parameter flow cytometry

Fluorescent staining techniques were used to study the anti-microbial properties of aqueous suspensions of a novel, water insoluble amino functionalised polymer on three micro-organisms Pseudomonas fluorescens, Staphylococcus epidermidis and Saccharomyses cerevisiae. The mechanism of action was simi...

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Bibliographic Details
Published in:Biotechnology letters 2004-04, Vol.26 (7), p.549-557
Main Authors: HEWITT, Christopher J, FRANKE, Robert, MARX, Achim, KOSSMANN, Beate, OTTERSBACH, Peter
Format: Article
Language:English
Subjects:
Amino Acids - pharmacology
Anti-Bacterial Agents - pharmacology
Antifungal Agents - pharmacology
Bacteria
Biological and medical sciences
Biotechnology
Cell Division - drug effects
Flow cytometry
Flow Cytometry - methods
Fundamental and applied biological sciences. Psychology
Methacrylates - pharmacology
Multivariate Analysis
Peptides
Polyamines
Polymers
Polymers - pharmacology
Pseudomonas fluorescens
Pseudomonas fluorescens - cytology
Pseudomonas fluorescens - drug effects
Saccharomyces cerevisiae
Saccharomyces cerevisiae - cytology
Saccharomyces cerevisiae - drug effects
Spectrometry, Fluorescence - methods
Staphylococcus epidermidis
Staphylococcus epidermidis - cytology
Staphylococcus epidermidis - drug effects
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Summary:Fluorescent staining techniques were used to study the anti-microbial properties of aqueous suspensions of a novel, water insoluble amino functionalised polymer on three micro-organisms Pseudomonas fluorescens, Staphylococcus epidermidis and Saccharomyses cerevisiae. The mechanism of action was similar for each organism in that, after various contact times with the polymer, a progressive change in individual cell physiological state was measured using multi-parameter flow cytometry. The microbiocidal activity of this polymer may be similar to that of substances referred to as polycationic, amphipathic compounds (peptides, peptide derivatives and other polyamines).
ISSN:0141-5492
1573-6776
DOI:10.1023/B:BILE.0000021954.82099.a0