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A study into the anti-microbial properties of an amino functionalised polymer using multi-parameter flow cytometry
Fluorescent staining techniques were used to study the anti-microbial properties of aqueous suspensions of a novel, water insoluble amino functionalised polymer on three micro-organisms Pseudomonas fluorescens, Staphylococcus epidermidis and Saccharomyses cerevisiae. The mechanism of action was simi...
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| Published in: | Biotechnology letters 2004-04, Vol.26 (7), p.549-557 |
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| Main Authors: | , , , , |
| Format: | Article |
| Language: | English |
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| cited_by | cdi_FETCH-LOGICAL-c440t-796eda178985a688cff3d230f1b78ec6809bce08c0eb58b721b37e2990c8acab3 |
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| container_end_page | 557 |
| container_issue | 7 |
| container_start_page | 549 |
| container_title | Biotechnology letters |
| container_volume | 26 |
| creator | HEWITT, Christopher J FRANKE, Robert MARX, Achim KOSSMANN, Beate OTTERSBACH, Peter |
| description | Fluorescent staining techniques were used to study the anti-microbial properties of aqueous suspensions of a novel, water insoluble amino functionalised polymer on three micro-organisms Pseudomonas fluorescens, Staphylococcus epidermidis and Saccharomyses cerevisiae. The mechanism of action was similar for each organism in that, after various contact times with the polymer, a progressive change in individual cell physiological state was measured using multi-parameter flow cytometry. The microbiocidal activity of this polymer may be similar to that of substances referred to as polycationic, amphipathic compounds (peptides, peptide derivatives and other polyamines). |
| doi_str_mv | 10.1023/B:BILE.0000021954.82099.a0 |
| format | article |
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The mechanism of action was similar for each organism in that, after various contact times with the polymer, a progressive change in individual cell physiological state was measured using multi-parameter flow cytometry. The microbiocidal activity of this polymer may be similar to that of substances referred to as polycationic, amphipathic compounds (peptides, peptide derivatives and other polyamines).</description><subject>Amino Acids - pharmacology</subject><subject>Anti-Bacterial Agents - pharmacology</subject><subject>Antifungal Agents - pharmacology</subject><subject>Bacteria</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Cell Division - drug effects</subject><subject>Flow cytometry</subject><subject>Flow Cytometry - methods</subject><subject>Fundamental and applied biological sciences. 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Psychology</topic><topic>Methacrylates - pharmacology</topic><topic>Multivariate Analysis</topic><topic>Peptides</topic><topic>Polyamines</topic><topic>Polymers</topic><topic>Polymers - pharmacology</topic><topic>Pseudomonas fluorescens</topic><topic>Pseudomonas fluorescens - cytology</topic><topic>Pseudomonas fluorescens - drug effects</topic><topic>Saccharomyces cerevisiae</topic><topic>Saccharomyces cerevisiae - cytology</topic><topic>Saccharomyces cerevisiae - drug effects</topic><topic>Spectrometry, Fluorescence - methods</topic><topic>Staphylococcus epidermidis</topic><topic>Staphylococcus epidermidis - cytology</topic><topic>Staphylococcus epidermidis - drug effects</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>HEWITT, Christopher J</creatorcontrib><creatorcontrib>FRANKE, Robert</creatorcontrib><creatorcontrib>MARX, Achim</creatorcontrib><creatorcontrib>KOSSMANN, Beate</creatorcontrib><creatorcontrib>OTTERSBACH, Peter</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Chemoreception Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>ProQuest_Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection (Proquest) (PQ_SDU_P3)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Engineering Collection</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>PML(ProQuest Medical Library)</collection><collection>ProQuest Science Journals</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Engineering Collection</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - 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The mechanism of action was similar for each organism in that, after various contact times with the polymer, a progressive change in individual cell physiological state was measured using multi-parameter flow cytometry. The microbiocidal activity of this polymer may be similar to that of substances referred to as polycationic, amphipathic compounds (peptides, peptide derivatives and other polyamines).</abstract><cop>Dordrecht</cop><pub>Springer</pub><pmid>15168853</pmid><doi>10.1023/B:BILE.0000021954.82099.a0</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0141-5492 |
| ispartof | Biotechnology letters, 2004-04, Vol.26 (7), p.549-557 |
| issn | 0141-5492 1573-6776 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_71972626 |
| source | Springer Nature |
| subjects | Amino Acids - pharmacology Anti-Bacterial Agents - pharmacology Antifungal Agents - pharmacology Bacteria Biological and medical sciences Biotechnology Cell Division - drug effects Flow cytometry Flow Cytometry - methods Fundamental and applied biological sciences. Psychology Methacrylates - pharmacology Multivariate Analysis Peptides Polyamines Polymers Polymers - pharmacology Pseudomonas fluorescens Pseudomonas fluorescens - cytology Pseudomonas fluorescens - drug effects Saccharomyces cerevisiae Saccharomyces cerevisiae - cytology Saccharomyces cerevisiae - drug effects Spectrometry, Fluorescence - methods Staphylococcus epidermidis Staphylococcus epidermidis - cytology Staphylococcus epidermidis - drug effects |
| title | A study into the anti-microbial properties of an amino functionalised polymer using multi-parameter flow cytometry |
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