Enzyme immunoassay for the diagnosis of brucellosis: chimeric Protein A–Protein G as a common enzyme labeled detection reagent for sera for different animal species

A recombinant protein combining the immunoglobulin binding sites of Proteins A and G, conjugated with horseradish peroxidase was used as a universal detection reagent for the assessment of antibodies against Brucella spp. The reagent was applied in an indirect enzyme immunoassay for detection of ant...

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Bibliographic Details
Published in:Veterinary microbiology 2004-06, Vol.101 (2), p.123-129
Main Authors: Nielsen, K., Smith, P., Yu, W., Nicoletti, P., Elzer, P., Vigliocco, A., Silva, P., Bermudez, R., Renteria, T., Moreno, F., Ruiz, A., Massengill, C., Muenks, Q., Kenny, K., Tollersrud, T., Samartino, L., Conde, S., Draghi de Benitez, G., Gall, D., Perez, B., Rojas, X.
Format: Article
Language:English
Subjects:
Animals
Antibodies, Bacterial - blood
Bacteriology
Biological and medical sciences
Brucella
Brucella - growth & development
Brucella spp
Brucellosis - blood
Brucellosis - diagnosis
Brucellosis - microbiology
Brucellosis - veterinary
Cattle
Dogs
Enzyme-Linked Immunosorbent Assay - methods
Enzyme-Linked Immunosorbent Assay - veterinary
False Negative Reactions
False Positive Reactions
Fundamental and applied biological sciences. Psychology
Goats
Indirect enzyme immunoassay
Lipopolysaccahride
Microbiology
Miscellaneous
Nerve Tissue Proteins - chemistry
Protein A/G
Recombinant Proteins - chemistry
Sensitivity and Specificity
Serology
Sheep
Staphylococcal Protein A - chemistry
Swine
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Description
Summary:A recombinant protein combining the immunoglobulin binding sites of Proteins A and G, conjugated with horseradish peroxidase was used as a universal detection reagent for the assessment of antibodies against Brucella spp. The reagent was applied in an indirect enzyme immunoassay for detection of antibodies to smooth lipopolysaccharide antigen in sera from Brucella spp. exposed and non-exposed cattle, sheep, goats and pigs and to antibodies to rough lipopolysaccharide in sheep, dogs and cattle. The results were similar to those obtained when murine monoclonal antibody–enzyme conjugates were used. An added advantage was that a universal cut-off for all tests using the proteins A and G detection reagent could be established, simplifying diagnostic interpretation of the data.
ISSN:0378-1135
1873-2542
DOI:10.1016/j.vetmic.2004.02.014