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Immunosuppressive and cytotoxic properties of Nigella sativa
In this study the volatile oil of Nigella sativa seeds (NSVO) was investigated for its immunomodulating and cytotoxic properties. A rat model was designed to examine the effect of NSVO on selected immune components. Long‐Evans rats were challenged with a specific antigen (typhoid TH) and treated with...
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| Published in: | Phytotherapy research 2004-05, Vol.18 (5), p.395-398 |
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| Main Authors: | , , , , |
| Format: | Article |
| Language: | English |
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| cited_by | cdi_FETCH-LOGICAL-c4099-bd35de97fd6834303a5dcd26961b9a551a0c5f99c36f1c8284823628249104143 |
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| cites | cdi_FETCH-LOGICAL-c4099-bd35de97fd6834303a5dcd26961b9a551a0c5f99c36f1c8284823628249104143 |
| container_end_page | 398 |
| container_issue | 5 |
| container_start_page | 395 |
| container_title | Phytotherapy research |
| container_volume | 18 |
| creator | Islam, S.N Begum, P Ahsan, T Huque, S Ahsan, M |
| description | In this study the volatile oil of Nigella sativa seeds (NSVO) was investigated for its immunomodulating and cytotoxic properties. A rat model was designed to examine the effect of NSVO on selected immune components. Long‐Evans rats were challenged with a specific antigen (typhoid TH) and treated with NSVO; and the changes produced in their serum antibody titre along with the splenocytes and peripheral immune cells were analysed. Antibody titre for the experimental animal was found to be 1280 as compared to the 2560 in the control rats. There was a significant (p < 0.05) decrease in splenocytes and neutrophils counts, but a rise in peripheral lymphocytes and monocytes in the experimental animals. To test the cytotoxicity of NSVO, a panel of five human cancer cell lines and a fibroblast line was used. The MTT assay was employed to estimate the cell mortality. Vinblastine sulphate and mitomycin C were used as the positive control. LC50 values for NSVO were 155.02 ± 10.4, 185.77 ± 2.9, 120.40 ± 20.5, 384.53 ± 12.1 and 286.83 ± 23.3 µg/ml respectively against the SCL, SCL‐6, SCL‐37′6, NUGC‐4 cancer lines and 3T6 fibroblast line. Results indicate that the NSVO could be considered as a potential immunosuppressive cytotoxic agent. Copyright © 2004 John Wiley & Sons, Ltd. |
| doi_str_mv | 10.1002/ptr.1449 |
| format | article |
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A rat model was designed to examine the effect of NSVO on selected immune components. Long‐Evans rats were challenged with a specific antigen (typhoid TH) and treated with NSVO; and the changes produced in their serum antibody titre along with the splenocytes and peripheral immune cells were analysed. Antibody titre for the experimental animal was found to be 1280 as compared to the 2560 in the control rats. There was a significant (p < 0.05) decrease in splenocytes and neutrophils counts, but a rise in peripheral lymphocytes and monocytes in the experimental animals. To test the cytotoxicity of NSVO, a panel of five human cancer cell lines and a fibroblast line was used. The MTT assay was employed to estimate the cell mortality. Vinblastine sulphate and mitomycin C were used as the positive control. LC50 values for NSVO were 155.02 ± 10.4, 185.77 ± 2.9, 120.40 ± 20.5, 384.53 ± 12.1 and 286.83 ± 23.3 µg/ml respectively against the SCL, SCL‐6, SCL‐37′6, NUGC‐4 cancer lines and 3T6 fibroblast line. Results indicate that the NSVO could be considered as a potential immunosuppressive cytotoxic agent. Copyright © 2004 John Wiley & Sons, Ltd.</description><identifier>ISSN: 0951-418X</identifier><identifier>EISSN: 1099-1573</identifier><identifier>DOI: 10.1002/ptr.1449</identifier><identifier>PMID: 15174000</identifier><language>eng</language><publisher>Chichester, UK: John Wiley & Sons, Ltd</publisher><subject>Animals ; Antineoplastic Agents, Phytogenic - administration & dosage ; Antineoplastic Agents, Phytogenic - pharmacology ; Antineoplastic Agents, Phytogenic - therapeutic use ; Biological and medical sciences ; Cell Line, Tumor - drug effects ; cytotoxicity ; Fibroblasts - drug effects ; functional foods ; General pharmacology ; Humans ; immunosuppressive agents ; Immunosuppressive Agents - administration & dosage ; Immunosuppressive Agents - pharmacology ; Immunosuppressive Agents - therapeutic use ; immunosuppressive cytotoxic agent ; Lymphocytes - drug effects ; Medical sciences ; medicinal plants ; Nigella sativa ; nutraceutical ; Pharmacognosy. Homeopathy. Health food ; Pharmacology. Drug treatments ; Phytotherapy ; Plant Oils - administration & dosage ; Plant Oils - pharmacology ; Plant Oils - therapeutic use ; Rats ; Rats, Long-Evans ; seed oils ; Spleen - cytology ; Spleen - drug effects</subject><ispartof>Phytotherapy research, 2004-05, Vol.18 (5), p.395-398</ispartof><rights>Copyright © 2004 John Wiley & Sons, Ltd.</rights><rights>2004 INIST-CNRS</rights><rights>Copyright 2004 John Wiley & Sons, Ltd.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4099-bd35de97fd6834303a5dcd26961b9a551a0c5f99c36f1c8284823628249104143</citedby><cites>FETCH-LOGICAL-c4099-bd35de97fd6834303a5dcd26961b9a551a0c5f99c36f1c8284823628249104143</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15778283$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15174000$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Islam, S.N</creatorcontrib><creatorcontrib>Begum, P</creatorcontrib><creatorcontrib>Ahsan, T</creatorcontrib><creatorcontrib>Huque, S</creatorcontrib><creatorcontrib>Ahsan, M</creatorcontrib><title>Immunosuppressive and cytotoxic properties of Nigella sativa</title><title>Phytotherapy research</title><addtitle>Phytother. Res</addtitle><description>In this study the volatile oil of Nigella sativa seeds (NSVO) was investigated for its immunomodulating and cytotoxic properties. A rat model was designed to examine the effect of NSVO on selected immune components. Long‐Evans rats were challenged with a specific antigen (typhoid TH) and treated with NSVO; and the changes produced in their serum antibody titre along with the splenocytes and peripheral immune cells were analysed. Antibody titre for the experimental animal was found to be 1280 as compared to the 2560 in the control rats. There was a significant (p < 0.05) decrease in splenocytes and neutrophils counts, but a rise in peripheral lymphocytes and monocytes in the experimental animals. To test the cytotoxicity of NSVO, a panel of five human cancer cell lines and a fibroblast line was used. The MTT assay was employed to estimate the cell mortality. Vinblastine sulphate and mitomycin C were used as the positive control. LC50 values for NSVO were 155.02 ± 10.4, 185.77 ± 2.9, 120.40 ± 20.5, 384.53 ± 12.1 and 286.83 ± 23.3 µg/ml respectively against the SCL, SCL‐6, SCL‐37′6, NUGC‐4 cancer lines and 3T6 fibroblast line. Results indicate that the NSVO could be considered as a potential immunosuppressive cytotoxic agent. Copyright © 2004 John Wiley & Sons, Ltd.</description><subject>Animals</subject><subject>Antineoplastic Agents, Phytogenic - administration & dosage</subject><subject>Antineoplastic Agents, Phytogenic - pharmacology</subject><subject>Antineoplastic Agents, Phytogenic - therapeutic use</subject><subject>Biological and medical sciences</subject><subject>Cell Line, Tumor - drug effects</subject><subject>cytotoxicity</subject><subject>Fibroblasts - drug effects</subject><subject>functional foods</subject><subject>General pharmacology</subject><subject>Humans</subject><subject>immunosuppressive agents</subject><subject>Immunosuppressive Agents - administration & dosage</subject><subject>Immunosuppressive Agents - pharmacology</subject><subject>Immunosuppressive Agents - therapeutic use</subject><subject>immunosuppressive cytotoxic agent</subject><subject>Lymphocytes - drug effects</subject><subject>Medical sciences</subject><subject>medicinal plants</subject><subject>Nigella sativa</subject><subject>nutraceutical</subject><subject>Pharmacognosy. Homeopathy. Health food</subject><subject>Pharmacology. Drug treatments</subject><subject>Phytotherapy</subject><subject>Plant Oils - administration & dosage</subject><subject>Plant Oils - pharmacology</subject><subject>Plant Oils - therapeutic use</subject><subject>Rats</subject><subject>Rats, Long-Evans</subject><subject>seed oils</subject><subject>Spleen - cytology</subject><subject>Spleen - drug effects</subject><issn>0951-418X</issn><issn>1099-1573</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><recordid>eNp10ctO3DAUBmCrAsEUkPoEbTZU3WTw8SWOpW5a1AEkRLnMCHaWx3GQIZkEO6Ezb4-jiVo2XVmWPp3z6z8IfQI8BYzJSdv5KTAmP6AJYClT4ILuoAmWHFIG-cM--hjCE8ZYEsz20D5wECx-J-j7RV33qyb0bettCO7VJnpVJGbTNV2zdiZpfdNa3zkbkqZMrtyjrSqdBN25V32IdktdBXs0vgdoMfs1Pz1PL3-fXZz-uEwNG9IsC8oLK0VZZDllFFPNC1OQTGawlJpz0NjwUkpDsxJMTnKWE5qRnDAJmAGjB-jrdm4M89Lb0KnaBTMEWdmmD0qAFBnGNMJvW2h8E4K3pWq9q7XfKMBqaErFptTQVKSfx5n9srbFPzhWE8HxCHQwuiq9XhkX3jkhYtRhZ7p1f1xlN_9dqK7nt-Pi0bvQ2fVfr_2zygQVXN1fnan858Pshs_v1Xn0X7a-1I3Sjz5mWNwRDDRekxNgQN8A-QaWXA</recordid><startdate>200405</startdate><enddate>200405</enddate><creator>Islam, S.N</creator><creator>Begum, P</creator><creator>Ahsan, T</creator><creator>Huque, S</creator><creator>Ahsan, M</creator><general>John Wiley & Sons, Ltd</general><general>Wiley</general><scope>FBQ</scope><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>200405</creationdate><title>Immunosuppressive and cytotoxic properties of Nigella sativa</title><author>Islam, S.N ; Begum, P ; Ahsan, T ; Huque, S ; Ahsan, M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4099-bd35de97fd6834303a5dcd26961b9a551a0c5f99c36f1c8284823628249104143</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Animals</topic><topic>Antineoplastic Agents, Phytogenic - administration & dosage</topic><topic>Antineoplastic Agents, Phytogenic - pharmacology</topic><topic>Antineoplastic Agents, Phytogenic - therapeutic use</topic><topic>Biological and medical sciences</topic><topic>Cell Line, Tumor - drug effects</topic><topic>cytotoxicity</topic><topic>Fibroblasts - drug effects</topic><topic>functional foods</topic><topic>General pharmacology</topic><topic>Humans</topic><topic>immunosuppressive agents</topic><topic>Immunosuppressive Agents - administration & dosage</topic><topic>Immunosuppressive Agents - pharmacology</topic><topic>Immunosuppressive Agents - therapeutic use</topic><topic>immunosuppressive cytotoxic agent</topic><topic>Lymphocytes - drug effects</topic><topic>Medical sciences</topic><topic>medicinal plants</topic><topic>Nigella sativa</topic><topic>nutraceutical</topic><topic>Pharmacognosy. Homeopathy. Health food</topic><topic>Pharmacology. Drug treatments</topic><topic>Phytotherapy</topic><topic>Plant Oils - administration & dosage</topic><topic>Plant Oils - pharmacology</topic><topic>Plant Oils - therapeutic use</topic><topic>Rats</topic><topic>Rats, Long-Evans</topic><topic>seed oils</topic><topic>Spleen - cytology</topic><topic>Spleen - drug effects</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Islam, S.N</creatorcontrib><creatorcontrib>Begum, P</creatorcontrib><creatorcontrib>Ahsan, T</creatorcontrib><creatorcontrib>Huque, S</creatorcontrib><creatorcontrib>Ahsan, M</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Phytotherapy research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Islam, S.N</au><au>Begum, P</au><au>Ahsan, T</au><au>Huque, S</au><au>Ahsan, M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Immunosuppressive and cytotoxic properties of Nigella sativa</atitle><jtitle>Phytotherapy research</jtitle><addtitle>Phytother. Res</addtitle><date>2004-05</date><risdate>2004</risdate><volume>18</volume><issue>5</issue><spage>395</spage><epage>398</epage><pages>395-398</pages><issn>0951-418X</issn><eissn>1099-1573</eissn><abstract>In this study the volatile oil of Nigella sativa seeds (NSVO) was investigated for its immunomodulating and cytotoxic properties. A rat model was designed to examine the effect of NSVO on selected immune components. Long‐Evans rats were challenged with a specific antigen (typhoid TH) and treated with NSVO; and the changes produced in their serum antibody titre along with the splenocytes and peripheral immune cells were analysed. Antibody titre for the experimental animal was found to be 1280 as compared to the 2560 in the control rats. There was a significant (p < 0.05) decrease in splenocytes and neutrophils counts, but a rise in peripheral lymphocytes and monocytes in the experimental animals. To test the cytotoxicity of NSVO, a panel of five human cancer cell lines and a fibroblast line was used. The MTT assay was employed to estimate the cell mortality. Vinblastine sulphate and mitomycin C were used as the positive control. LC50 values for NSVO were 155.02 ± 10.4, 185.77 ± 2.9, 120.40 ± 20.5, 384.53 ± 12.1 and 286.83 ± 23.3 µg/ml respectively against the SCL, SCL‐6, SCL‐37′6, NUGC‐4 cancer lines and 3T6 fibroblast line. Results indicate that the NSVO could be considered as a potential immunosuppressive cytotoxic agent. Copyright © 2004 John Wiley & Sons, Ltd.</abstract><cop>Chichester, UK</cop><pub>John Wiley & Sons, Ltd</pub><pmid>15174000</pmid><doi>10.1002/ptr.1449</doi><tpages>4</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0951-418X |
| ispartof | Phytotherapy research, 2004-05, Vol.18 (5), p.395-398 |
| issn | 0951-418X 1099-1573 |
| language | eng |
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| source | Wiley-Blackwell Read & Publish Collection |
| subjects | Animals Antineoplastic Agents, Phytogenic - administration & dosage Antineoplastic Agents, Phytogenic - pharmacology Antineoplastic Agents, Phytogenic - therapeutic use Biological and medical sciences Cell Line, Tumor - drug effects cytotoxicity Fibroblasts - drug effects functional foods General pharmacology Humans immunosuppressive agents Immunosuppressive Agents - administration & dosage Immunosuppressive Agents - pharmacology Immunosuppressive Agents - therapeutic use immunosuppressive cytotoxic agent Lymphocytes - drug effects Medical sciences medicinal plants Nigella sativa nutraceutical Pharmacognosy. Homeopathy. Health food Pharmacology. Drug treatments Phytotherapy Plant Oils - administration & dosage Plant Oils - pharmacology Plant Oils - therapeutic use Rats Rats, Long-Evans seed oils Spleen - cytology Spleen - drug effects |
| title | Immunosuppressive and cytotoxic properties of Nigella sativa |
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