Sodium bicarbonate treatment and ubiquitin gene expression in acidotic human subjects with chronic renal failure

Background In chronic renal failure, metabolic acidosis is associated with increased whole body protein degradation. In rats this effect of acidosis occurs in skeletal muscle and is associated with increased ubiquitin mRNA expression. This has not been demonstrated in humans. Materials and methods S...

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Bibliographic Details
Published in:European journal of clinical investigation 2002-07, Vol.32 (7), p.488-492
Main Authors: Roberts, R. G., Redfern, C. P. F., Graham, K. A., Bartlett, K., Wilkinson, R., Goodship, T. H. J.
Format: Article
Language:English
Subjects:
Acidosis - drug therapy
Adult
Biological and medical sciences
Biopsy
Blotting, Northern - methods
Chronic renal failure
Complement C2 - genetics
Female
Gene Expression
Humans
Kidney Failure, Chronic - drug therapy
Kidney Failure, Chronic - metabolism
Male
Medical sciences
metabolic acidosis
Middle Aged
Muscle, Skeletal - metabolism
Nephrology. Urinary tract diseases
Nephropathies. Renovascular diseases. Renal failure
protein degradation
Renal failure
RNA - analysis
Sodium Bicarbonate - metabolism
Sodium Bicarbonate - therapeutic use
Treatment Failure
ubiquitin
Ubiquitin - genetics
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Summary:Background In chronic renal failure, metabolic acidosis is associated with increased whole body protein degradation. In rats this effect of acidosis occurs in skeletal muscle and is associated with increased ubiquitin mRNA expression. This has not been demonstrated in humans. Materials and methods Six patients with chronic renal failure and acidosis underwent muscle biopsy before and after 1 month's treatment with sodium bicarbonate. RNA was extracted from the biopsy, and the expression of the genes for ubiquitin and the proteasome component, C2, were measured by Northern blotting. Results and conclusions There was no significant difference in the expression of ubiquitin or C2 after bicarbonate treatment. This is contrast with results from animal models of acidosis and some other catabolic conditions in humans. This may reflect the complexity of the ubiquitin‐dependent pathway, and it may be that changes in ubiquitin expression are only seen with more severe and/or acute changes in pH.
ISSN:0014-2972
1365-2362
DOI:10.1046/j.1365-2362.2002.01008.x