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Reduction in the Circulating pDC1/pDC2 Ratio and Impaired Function of Ex Vivo-Generated DC1 In Chronic Hepatitis B Infection
Dendritic cells (DCs) induce and regulate T-cell-mediated immune responses. Circulating precursor (p)DC1 and pDC2 from patients with chronic hepatitis B virus (HBV) infection were quantified by flow cytometry. To assess their function, DC1 were cultured from patients and compared to those of healthy...
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| Published in: | Clinical immunology (Orlando, Fla.) Fla.), 2002-08, Vol.104 (2), p.138-150 |
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| Main Authors: | , , , , , , , , , , |
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| cited_by | cdi_FETCH-LOGICAL-c467t-b3eaa66aab66ec87cc9c50b18ca288df1435d808cf77de865808eff563a28e593 |
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| cites | cdi_FETCH-LOGICAL-c467t-b3eaa66aab66ec87cc9c50b18ca288df1435d808cf77de865808eff563a28e593 |
| container_end_page | 150 |
| container_issue | 2 |
| container_start_page | 138 |
| container_title | Clinical immunology (Orlando, Fla.) |
| container_volume | 104 |
| creator | Beckebaum, Susanne Cicinnati, Vito R. Dworacki, Grzegorz Müller-Berghaus, Jan Stolz, Donna Harnaha, Jo Whiteside, Theresa L. Thomson, Angus W. Lu, Lina Fung, John J. Bonham, C.Andrew |
| description | Dendritic cells (DCs) induce and regulate T-cell-mediated immune responses. Circulating precursor (p)DC1 and pDC2 from patients with chronic hepatitis B virus (HBV) infection were quantified by flow cytometry. To assess their function, DC1 were cultured from patients and compared to those of healthy volunteers. HBV patients exhibited a significant decrease in the proportion of freshly isolated pDC1 to pDC2. DC1 propagated from patients showed lower expression of costimulatory molecules and impaired allostimulatory capacity in comparison to controls. After exposure to proinflammatory cytokines, expression of costimulatory molecules, secretion of interleukin-12 (IL-12) and allostimulatory properties increased, but capacity for T-cell stimulation and IL-12 production remained inferior to that of control DCs. HBV-DNA was amplified by polymerase chain reaction in DC1 cultured from all patients. Viral particles were visible in DC1 by electron microscopy. These results suggest that intracellular presence of HBV impairs DC1 functional maturation and subsequent deficits in T-lymphocyte activation may contribute to viral persistence. |
| doi_str_mv | 10.1006/clim.2002.5245 |
| format | article |
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Circulating precursor (p)DC1 and pDC2 from patients with chronic hepatitis B virus (HBV) infection were quantified by flow cytometry. To assess their function, DC1 were cultured from patients and compared to those of healthy volunteers. HBV patients exhibited a significant decrease in the proportion of freshly isolated pDC1 to pDC2. DC1 propagated from patients showed lower expression of costimulatory molecules and impaired allostimulatory capacity in comparison to controls. After exposure to proinflammatory cytokines, expression of costimulatory molecules, secretion of interleukin-12 (IL-12) and allostimulatory properties increased, but capacity for T-cell stimulation and IL-12 production remained inferior to that of control DCs. HBV-DNA was amplified by polymerase chain reaction in DC1 cultured from all patients. Viral particles were visible in DC1 by electron microscopy. 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Circulating precursor (p)DC1 and pDC2 from patients with chronic hepatitis B virus (HBV) infection were quantified by flow cytometry. To assess their function, DC1 were cultured from patients and compared to those of healthy volunteers. HBV patients exhibited a significant decrease in the proportion of freshly isolated pDC1 to pDC2. DC1 propagated from patients showed lower expression of costimulatory molecules and impaired allostimulatory capacity in comparison to controls. After exposure to proinflammatory cytokines, expression of costimulatory molecules, secretion of interleukin-12 (IL-12) and allostimulatory properties increased, but capacity for T-cell stimulation and IL-12 production remained inferior to that of control DCs. HBV-DNA was amplified by polymerase chain reaction in DC1 cultured from all patients. Viral particles were visible in DC1 by electron microscopy. These results suggest that intracellular presence of HBV impairs DC1 functional maturation and subsequent deficits in T-lymphocyte activation may contribute to viral persistence.</description><subject>allostimulation</subject><subject>antigen presentation</subject><subject>Biological and medical sciences</subject><subject>Cell Division</subject><subject>Cells, Cultured</subject><subject>Dendritic Cells - drug effects</subject><subject>Dendritic Cells - immunology</subject><subject>Dendritic Cells - virology</subject><subject>DNA, Viral - analysis</subject><subject>DNA, Viral - blood</subject><subject>Female</subject><subject>Granulocyte-Macrophage Colony-Stimulating Factor - pharmacology</subject><subject>Hepatitis B virus - genetics</subject><subject>Hepatitis B virus - isolation & purification</subject><subject>Hepatitis B, Chronic - blood</subject><subject>Hepatitis B, Chronic - immunology</subject><subject>Human viral diseases</subject><subject>Humans</subject><subject>IFN-γ</subject><subject>IL-12</subject><subject>Immunophenotyping</subject><subject>Infectious diseases</subject><subject>Interleukin-12 - analysis</subject><subject>Interleukin-4 - pharmacology</subject><subject>liver</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Middle Aged</subject><subject>Time Factors</subject><subject>Viral diseases</subject><subject>Viral hepatitis</subject><subject>viral infection</subject><issn>1521-6616</issn><issn>1521-7035</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><recordid>eNqFkUtv1TAQhS0EoqWwZYm8gV1ubSd-ZAmhjytVQqqAreVrj6lR4gQ7qUDqj6_DjdQVYmOPZr5zxvJB6C0lO0qIOLd9GHaMELbjrOHP0CnljFaS1Pz5VgtBxQl6lfNPQghnTLxEJ5RRwZnkp-jhFtxi5zBGHCKe7wB3IdmlN3OIP_D0uaPn5WD4tjRGbKLD-2EyIYHDl0s8CkePL37j7-F-rK4gQjJzmRYl3kfc3aUxBouvYSoOc8j4U2l7-Kt8jV5402d4s91n6Nvlxdfuurr5crXvPt5UthFyrg41GCOEMQchwCppbWs5OVBlDVPKedrU3CmirJfSgRK81OA9F3WZA2_rM_Th6Dul8dcCedZDyBb63kQYl6wlbWUrBfsvSBUnpG2aAu6OoE1jzgm8nlIYTPqjKdFrMHoNRq_B6DWYIni3OS-HAdwTviVRgPcbYLI1vU8m2pCfuLqsVXTdrI4clA-7D5B0tgGiBVdCsbN2Y_jXGx4BCeqokQ</recordid><startdate>20020801</startdate><enddate>20020801</enddate><creator>Beckebaum, Susanne</creator><creator>Cicinnati, Vito R.</creator><creator>Dworacki, Grzegorz</creator><creator>Müller-Berghaus, Jan</creator><creator>Stolz, Donna</creator><creator>Harnaha, Jo</creator><creator>Whiteside, Theresa L.</creator><creator>Thomson, Angus W.</creator><creator>Lu, Lina</creator><creator>Fung, John J.</creator><creator>Bonham, C.Andrew</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>20020801</creationdate><title>Reduction in the Circulating pDC1/pDC2 Ratio and Impaired Function of Ex Vivo-Generated DC1 In Chronic Hepatitis B Infection</title><author>Beckebaum, Susanne ; Cicinnati, Vito R. ; Dworacki, Grzegorz ; Müller-Berghaus, Jan ; Stolz, Donna ; Harnaha, Jo ; Whiteside, Theresa L. ; Thomson, Angus W. ; Lu, Lina ; Fung, John J. ; Bonham, C.Andrew</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c467t-b3eaa66aab66ec87cc9c50b18ca288df1435d808cf77de865808eff563a28e593</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>allostimulation</topic><topic>antigen presentation</topic><topic>Biological and medical sciences</topic><topic>Cell Division</topic><topic>Cells, Cultured</topic><topic>Dendritic Cells - drug effects</topic><topic>Dendritic Cells - immunology</topic><topic>Dendritic Cells - virology</topic><topic>DNA, Viral - analysis</topic><topic>DNA, Viral - blood</topic><topic>Female</topic><topic>Granulocyte-Macrophage Colony-Stimulating Factor - pharmacology</topic><topic>Hepatitis B virus - genetics</topic><topic>Hepatitis B virus - isolation & purification</topic><topic>Hepatitis B, Chronic - blood</topic><topic>Hepatitis B, Chronic - immunology</topic><topic>Human viral diseases</topic><topic>Humans</topic><topic>IFN-γ</topic><topic>IL-12</topic><topic>Immunophenotyping</topic><topic>Infectious diseases</topic><topic>Interleukin-12 - analysis</topic><topic>Interleukin-4 - pharmacology</topic><topic>liver</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Middle Aged</topic><topic>Time Factors</topic><topic>Viral diseases</topic><topic>Viral hepatitis</topic><topic>viral infection</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Beckebaum, Susanne</creatorcontrib><creatorcontrib>Cicinnati, Vito R.</creatorcontrib><creatorcontrib>Dworacki, Grzegorz</creatorcontrib><creatorcontrib>Müller-Berghaus, Jan</creatorcontrib><creatorcontrib>Stolz, Donna</creatorcontrib><creatorcontrib>Harnaha, Jo</creatorcontrib><creatorcontrib>Whiteside, Theresa L.</creatorcontrib><creatorcontrib>Thomson, Angus W.</creatorcontrib><creatorcontrib>Lu, Lina</creatorcontrib><creatorcontrib>Fung, John J.</creatorcontrib><creatorcontrib>Bonham, C.Andrew</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Clinical immunology (Orlando, Fla.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Beckebaum, Susanne</au><au>Cicinnati, Vito R.</au><au>Dworacki, Grzegorz</au><au>Müller-Berghaus, Jan</au><au>Stolz, Donna</au><au>Harnaha, Jo</au><au>Whiteside, Theresa L.</au><au>Thomson, Angus W.</au><au>Lu, Lina</au><au>Fung, John J.</au><au>Bonham, C.Andrew</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Reduction in the Circulating pDC1/pDC2 Ratio and Impaired Function of Ex Vivo-Generated DC1 In Chronic Hepatitis B Infection</atitle><jtitle>Clinical immunology (Orlando, Fla.)</jtitle><addtitle>Clin Immunol</addtitle><date>2002-08-01</date><risdate>2002</risdate><volume>104</volume><issue>2</issue><spage>138</spage><epage>150</epage><pages>138-150</pages><issn>1521-6616</issn><eissn>1521-7035</eissn><coden>CLIIFY</coden><abstract>Dendritic cells (DCs) induce and regulate T-cell-mediated immune responses. Circulating precursor (p)DC1 and pDC2 from patients with chronic hepatitis B virus (HBV) infection were quantified by flow cytometry. To assess their function, DC1 were cultured from patients and compared to those of healthy volunteers. HBV patients exhibited a significant decrease in the proportion of freshly isolated pDC1 to pDC2. DC1 propagated from patients showed lower expression of costimulatory molecules and impaired allostimulatory capacity in comparison to controls. After exposure to proinflammatory cytokines, expression of costimulatory molecules, secretion of interleukin-12 (IL-12) and allostimulatory properties increased, but capacity for T-cell stimulation and IL-12 production remained inferior to that of control DCs. HBV-DNA was amplified by polymerase chain reaction in DC1 cultured from all patients. Viral particles were visible in DC1 by electron microscopy. These results suggest that intracellular presence of HBV impairs DC1 functional maturation and subsequent deficits in T-lymphocyte activation may contribute to viral persistence.</abstract><cop>San Diego, CA</cop><pub>Elsevier Inc</pub><pmid>12165275</pmid><doi>10.1006/clim.2002.5245</doi><tpages>13</tpages></addata></record> |
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| ispartof | Clinical immunology (Orlando, Fla.), 2002-08, Vol.104 (2), p.138-150 |
| issn | 1521-6616 1521-7035 |
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| subjects | allostimulation antigen presentation Biological and medical sciences Cell Division Cells, Cultured Dendritic Cells - drug effects Dendritic Cells - immunology Dendritic Cells - virology DNA, Viral - analysis DNA, Viral - blood Female Granulocyte-Macrophage Colony-Stimulating Factor - pharmacology Hepatitis B virus - genetics Hepatitis B virus - isolation & purification Hepatitis B, Chronic - blood Hepatitis B, Chronic - immunology Human viral diseases Humans IFN-γ IL-12 Immunophenotyping Infectious diseases Interleukin-12 - analysis Interleukin-4 - pharmacology liver Male Medical sciences Middle Aged Time Factors Viral diseases Viral hepatitis viral infection |
| title | Reduction in the Circulating pDC1/pDC2 Ratio and Impaired Function of Ex Vivo-Generated DC1 In Chronic Hepatitis B Infection |
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