Multielement trace-element speciation in metal-biomolecules by chromatography coupled with ICP-MS

The state-of-the-art of multi-element speciation in biological materials by hybrid techniques with inductively coupled plasma-mass spectrometric (ICP-MS) detection is critically reviewed with special attention to multi-metal speciation in metallothioneins (MT) and metallothionein-like proteins (MLP)...

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Bibliographic Details
Published in:Analytical and bioanalytical chemistry 2002-07, Vol.373 (6), p.412-421
Main Authors: Ferrarello, C N, Fernández de la Campa, M R, Sanz-Medel, A
Format: Article
Language:English
Subjects:
Biochemistry - methods
Chromatography - methods
Mass Spectrometry - methods
Metallothionein - chemistry
Metallothionein - isolation & purification
Metals - analysis
Trace Elements - analysis
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Summary:The state-of-the-art of multi-element speciation in biological materials by hybrid techniques with inductively coupled plasma-mass spectrometric (ICP-MS) detection is critically reviewed with special attention to multi-metal speciation in metallothioneins (MT) and metallothionein-like proteins (MLP). The most common separation techniques used for the purpose include conventional size-exclusion chromatography (SEC) (with off-line detection) and anion-exchange (AE) or reversed-phase (RP) HPLC coupled on-line to ICP-MS. Advantages and limitations of the different commercial element-selective ICP-MS detectors, including the quadrupole (Q), double focusing (DF), and time-of-flight (TOF) mass analysers, are discussed. Procedures reported for speciation analysis, by use of hybrid techniques, for multi-metal speciation in mussel MLP and rabbit liver MT are illustrated with special emphasis on work performed in the authors' laboratory.
ISSN:1618-2642
1618-2650
DOI:10.1007/s00216-002-1278-y