Nucleolar asynchrony observed in HL-60 leukemic granulocytic precursors resistant to 5-aminolaevulinic acid-based photodynamic treatment

To provide more information on the 5-aminolaevulinic acid (ALA)-induced photodynamic effect on nucleoli, morphologically expressed nucleolar asynchrony (the presence of ‘active’ large nucleoli with an uniform distribution of RNA and ‘resting’ ring-shaped nucleoli in one and the same nucleus) was stu...

Full description

Saved in:
Bibliographic Details
Published in:Journal of photochemistry and photobiology. B, Biology Biology, 2002-07, Vol.67 (3), p.201-203
Main Authors: Smetana, Karel, Cajthamlová, Hana, Grebeňová, Dana, Jirásková, Ilona, Hrkal, Zbyněk
Format: Article
Language:English
Subjects:
5-Aminolaevulinic acid
Aminolevulinic Acid - pharmacology
Cell Nucleolus - drug effects
Cell Nucleolus - ultrastructure
HL-60 Cells
Humans
Nucleolar asynchrony
Photochemotherapy
Photodynamic effect
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:To provide more information on the 5-aminolaevulinic acid (ALA)-induced photodynamic effect on nucleoli, morphologically expressed nucleolar asynchrony (the presence of ‘active’ large nucleoli with an uniform distribution of RNA and ‘resting’ ring-shaped nucleoli in one and the same nucleus) was studied in cultured HL-60 leukemic granulocytic precursors using a simple cytochemical procedure for the demonstration of RNA. Nucleolar asynchrony was mainly expressed in cells which were apparently resistant to ALA-based photodynamic treatment (PDT) since most of them (about 75%) exhibited this phenomenon.
ISSN:1011-1344
1873-2682
DOI:10.1016/S1011-1344(02)00307-X