Isolation of high quality RNA from Streptomyces

Several molecular techniques require high quality RNA, completely free of DNA. Standard methods to isolate total RNA from Streptomyces spp. are based on the application of a ‘Modified Kirby Mix’ [Practical Streptomyces Genetics. The John Innes Foundation, Norwich, pp. 613]. Here we present an altern...

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Bibliographic Details
Published in:Journal of microbiological methods 2004-07, Vol.58 (1), p.135-137
Main Authors: Van Dessel, W, Van Mellaert, L, Geukens, N, Lammertyn, E, Anné, J
Format: Article
Language:English
Subjects:
Bacteriological methods and techniques used in bacteriology
Bacteriology
Biological and medical sciences
Edetic Acid - chemistry
Fundamental and applied biological sciences. Psychology
Guanidines - chemistry
Microbiology
Octoxynol - chemistry
Phenols - chemistry
RNA isolation
RNA, Bacterial - isolation & purification
Streptomyces
Streptomyces - chemistry
Streptomyces - genetics
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Summary:Several molecular techniques require high quality RNA, completely free of DNA. Standard methods to isolate total RNA from Streptomyces spp. are based on the application of a ‘Modified Kirby Mix’ [Practical Streptomyces Genetics. The John Innes Foundation, Norwich, pp. 613]. Here we present an alternative procedure using Triton® X-100 and EDTA for the isolation of total RNA from Streptomyces.
ISSN:0167-7012
1872-8359
DOI:10.1016/j.mimet.2004.03.015