Leukocyte mitochondrial alterations after cardiac surgery involving cardiopulmonary bypass: Clinical correlations

Cardiac surgery with the use of cardiopulmonary bypass (CPB) is known to initiate systemic inflammatory responses that are associated with immune dysregulations, but the pathomechanisms underlying these changes remain elusive. Mitochondrial transmembrane potential (MTP) is an important determinant o...

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Bibliographic Details
Published in:Shock (Augusta, Ga.) Ga.), 2004-04, Vol.21 (4), p.315-319
Main Authors: KONG, Chi-Woon, HUANG, Cheng-Hsiung, HSU, Tai-Ger, TSAI, Kelvin K. C, HSU, Chen-Fu, HUANG, Mei-Chun, CHEN, Lung-Ching
Format: Article
Language:English
Subjects:
Aged
Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy
Biological and medical sciences
Biomarkers - blood
Cardiopulmonary Bypass - adverse effects
Cell Survival
Female
Humans
Intensive care medicine
Leukocytes - pathology
Male
Medical sciences
Membrane Potentials - physiology
Mitochondria - metabolism
Mitochondria - pathology
Nitric Oxide - blood
Nitric Oxide - metabolism
Superoxides - metabolism
Thoracic Surgery
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Summary:Cardiac surgery with the use of cardiopulmonary bypass (CPB) is known to initiate systemic inflammatory responses that are associated with immune dysregulations, but the pathomechanisms underlying these changes remain elusive. Mitochondrial transmembrane potential (MTP) is an important determinant of leukocytic functions and viability, and may be altered as a part of the cellular responses to systemic inflammatory insults. Therefore, we examined MTP in three subsets of peripheral leukocytes in 18 patients receiving uncomplicated cardiac surgery involving CPB. The MTP of neutrophils and lymphocytes significantly increased, whereas that of monocytes significantly declined, after the surgery. The alterations in leukocytic MTP were transient, normalizing 3 days to 1 week after the surgery, and were accompanied by transient overproduction of intracellular oxidants, including nitric oxide and superoxide. Despite these perturbations, the viability status of leukocytes remained unaltered. Positive correlations were found between the changes of leukocyte MTP and various clinical parameters, implying that leukocyte mitochondrial alterations are parts of the systemic immune perturbations induced by the bypass surgery.
ISSN:1073-2322
1540-0514
DOI:10.1097/00024382-200404000-00005