EXPLANT-CELL CULTURE OF PRIMARY MAMMARY TUMORS FROM MMTV-c-Myc TRANSGENIC MICE

We have established an explant-cell culture system for mammary gland tumors from c-myc oncogene–expressing transgenic mice and potentially other transgenic strains. By coating culture dish surfaces with fetal bovine serum and using culture media supplemented with low serum and growth factors, the ma...

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Bibliographic Details
Published in:In vitro cellular & developmental biology. Animal 2004-01, Vol.40 (1), p.14-21
Main Authors: PEI, XU FANG, NOBLE, MARCIA S, DAVOLI, MARIA ANTONIETTA, ROSFJORD, EDWARD, TILLI, MADDALENA T, FURTH, PRISCILLA A, RUSSELL, ROBERT, JOHNSON, MICHAEL D, DICKSON, ROBERT B
Format: Article
Language:English
Subjects:
Animals
B lymphocytes
Breast Neoplasms - genetics
Breast Neoplasms - metabolism
Breast Neoplasms - pathology
Cattle
CELL AND TISSUE MODELS
Cell Culture Techniques
Cell growth
Cell lines
Cell Shape
Culture Media - chemistry
Cultured tumor cells
differentiation
Epithelial cells
epithelial culture
Female
gelatin
Humans
Keratins - metabolism
Mammary glands
Mammary Tumor Virus, Mouse - genetics
Mammary Tumor Virus, Mouse - metabolism
Mice
Mice, Transgenic
Proto-Oncogene Proteins c-myc - genetics
Proto-Oncogene Proteins c-myc - metabolism
Salivary Gland Neoplasms - metabolism
Salivary Gland Neoplasms - pathology
serial outgrowth
serum
Spleen cells
Transgenic animals
Tumor cell line
Tumor Cells, Cultured
Tumors
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Description
Summary:We have established an explant-cell culture system for mammary gland tumors from c-myc oncogene–expressing transgenic mice and potentially other transgenic strains. By coating culture dish surfaces with fetal bovine serum and using culture media supplemented with low serum and growth factors, the mammary tumor specimens could be maintained in culture for over 3 mo. Throughout the culture period, the explants produced abundant outgrowths of epithelial cells. As the outgrowths of epithelial cells filled the dishes, the explants were serially transferred from one dish to another—a process that could be repeated at least six times, thus providing a continuous supply of primary tumor cells. This culture system provides a useful tool for studying the biology of mouse mammary gland tumors and possibly tumors from other organ sites.
ISSN:1071-2690
1543-706X
1543-706X
DOI:10.1290/1543-706X(2004)40<14:ECOPMT>2.0.CO;2