Differential migratory properties of mouse, fish, and frog leukocytes treated with agonists of opioid receptors

Zymosan-induced peritoneal inflammation was inhibited by morphine co-injection in mice and fish but not in anuran amphibians. In present experiments, an in vitro migration of mouse, goldfish, and frog leukocytes to L15 medium, control serum (S) or zymosan-activated serum (ZAS) was recorded following...

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Bibliographic Details
Published in:Developmental and comparative immunology 2004-07, Vol.28 (9), p.949-958
Main Authors: Chadzinska, Magdalena, Plytycz, Barbara
Format: Article
Language:English
Subjects:
3,4-Dichloro-N-methyl-N-(2-(1-pyrrolidinyl)-cyclohexyl)-benzeneacetamide, (trans)-Isomer - pharmacology
Analgesics, Non-Narcotic - pharmacology
Analgesics, Opioid - pharmacology
Animals
Anura
Carassius auratus
Chemotaxis
Chemotaxis, Leukocyte - drug effects
Chemotaxis, Leukocyte - physiology
Culture Media, Serum-Free
Enzyme Precursors - pharmacology
Goldfish - immunology
Inflammation
Leukocytes
Mice - immunology
Migration
Morphine - pharmacology
Oligopeptides - pharmacology
Opioids
Organic Chemicals
Rana esculenta
Rana esculenta - immunology
Receptors, Opioid - drug effects
Receptors, Opioid - metabolism
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Summary:Zymosan-induced peritoneal inflammation was inhibited by morphine co-injection in mice and fish but not in anuran amphibians. In present experiments, an in vitro migration of mouse, goldfish, and frog leukocytes to L15 medium, control serum (S) or zymosan-activated serum (ZAS) was recorded following cell preincubation with L15 or with agonists of mu, delta, or kappa opioid receptors (morphine, deltorphine, or U-50,488H, respectively). In all species, migration of control leukocytes was in the order ZAS>S>L15. Pretreatment with morphine or deltorphine (but not with U-50,488H) enhanced leukocyte migration to L15 and S in each species, while it inhibited migration of mouse and fish (but not frog) leukocytes to ZAS, phenomena reversed by specific antagonists of mu and delta opioid receptors (CTOP or naltrindole, respectively). It seems that final effects of opioids on cell migration are dependent on a species-specific balance between up- and down-regulation of leukocyte migration resulted from interplay between receptors for opioids and chemotactic factors.
ISSN:0145-305X
1879-0089
DOI:10.1016/j.dci.2004.02.003