Rapid detection of quinolone-resistant Salmonella by real time SNP genotyping

A total of 63 isolates were screened for the gyrA mutation ( 87Asp–Tyr) in Salmonella enterica serovars using real time PCR. All of the isolates were successfully identified as resistant or susceptible, consistent with the MIC result of the agar dilution method and gyrA sequencing.

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Bibliographic Details
Published in:Journal of microbiological methods 2004-07, Vol.58 (1), p.131-134
Main Authors: Esaki, Hidetake, Noda, Ken, Otsuki, Noriyuki, Kojima, Akemi, Asai, Tetsuo, Tamura, Yutaka, Takahashi, Toshio
Format: Article
Language:English
Subjects:
Animals
Anti-Infective Agents - pharmacology
Bacteriological methods and techniques used in bacteriology
Bacteriology
Biological and medical sciences
Cattle
Cattle Diseases - microbiology
Cycling probe
DNA Gyrase - chemistry
DNA Gyrase - genetics
DNA, Bacterial - chemistry
DNA, Bacterial - genetics
Drug Resistance, Bacterial
Fluorescent Dyes - chemistry
Fundamental and applied biological sciences. Psychology
Microbial Sensitivity Tests
Microbiology
Polymerase Chain Reaction - methods
Polymorphism, Single Nucleotide
Quinolone-resistant Salmonella
Quinolones - pharmacology
Rapid detection
Real time PCR
Salmonella enterica
Salmonella enterica - drug effects
Salmonella enterica - genetics
Salmonella enterica - isolation & purification
Salmonella Infections, Animal - microbiology
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Description
Summary:A total of 63 isolates were screened for the gyrA mutation ( 87Asp–Tyr) in Salmonella enterica serovars using real time PCR. All of the isolates were successfully identified as resistant or susceptible, consistent with the MIC result of the agar dilution method and gyrA sequencing.
ISSN:0167-7012
1872-8359
DOI:10.1016/j.mimet.2004.03.010