Separation and Characterization of Phenolic Compounds in Fennel (Foeniculum vulgare) Using Liquid Chromatography−Negative Electrospray Ionization Tandem Mass Spectrometry

Liquid chromatography (LC) diode array detection (DAD) coupled to negative electrospray ionization (ESI) tandem mass spectrometry (MS/MS) was used for the rapid and sensitive identification of water-soluble phenolic compounds in fennel waste. The plant material was first extracted and then chromatog...

Full description

Saved in:
Bibliographic Details
Published in:Journal of agricultural and food chemistry 2004-06, Vol.52 (12), p.3679-3687
Main Authors: Parejo, Irene, Jauregui, Olga, Sánchez-Rabaneda, Ferran, Viladomat, Francesc, Bastida, Jaume, Codina, Carles
Format: Article
Language:English
Subjects:
Biological and medical sciences
Chemical Fractionation
Chromatography, Liquid
Coumaric Acids - analysis
Coumaric Acids - isolation & purification
Flavonoids - analysis
Flavonoids - isolation & purification
Foeniculum - chemistry
Food industries
Fruit and vegetable industries
Fundamental and applied biological sciences. Psychology
Glycosides - analysis
Glycosides - isolation & purification
Phenols - analysis
Phenols - isolation & purification
Spectrometry, Mass, Electrospray Ionization
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Liquid chromatography (LC) diode array detection (DAD) coupled to negative electrospray ionization (ESI) tandem mass spectrometry (MS/MS) was used for the rapid and sensitive identification of water-soluble phenolic compounds in fennel waste. The plant material was first extracted and then chromatographed on Sephadex LH-20 to afford seven fractions, each of them being subjected to LC-MS analysis. Identification of the compounds was carried out by interpretation of UV, MS, and MS/MS spectra. Forty-two phenolic substances were identified, 27 of which had not previously been reported in fennel, including hydroxycinnamic acid derivatives, flavonoid glycosides, and flavonoid aglycons. Keywords: Fennel; Foeniculum vulgare; tandem mass spectrometry; flavonoids; phenolics; liquid chromatography
ISSN:0021-8561
1520-5118
DOI:10.1021/jf030813h