Cellular and molecular targets of estrogen in normal human breast tissue

To gain insight into the in vivo role of estrogen, we isolated estrogen receptor-positive cells fromnormal human breast tissue using a recombinant adenovirus that expresses green fluorescence protein in response to estrogen. We compared the global gene expression profile of these estrogen receptor-p...

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Bibliographic Details
Published in:Cancer research (Chicago, Ill.) Ill.), 2002-08, Vol.62 (16), p.4540-4544
Main Authors: SETH, Pankaj, PORTER, Dale, LAHTI-DOMENICI, Jaana, YAN GENG, RICHARDSON, Andrea, POLYAK, Kornelia
Format: Article
Language:English
Subjects:
Adenoviridae - genetics
Adolescent
Adult
Biological and medical sciences
Breast - metabolism
Breast - physiology
Breast Neoplasms - genetics
Breast Neoplasms - metabolism
Carrier Proteins - biosynthesis
Carrier Proteins - genetics
Estrogens - pharmacology
Estrogens - physiology
Female
Gene Expression Profiling
Green Fluorescent Proteins
Gynecology. Andrology. Obstetrics
Humans
Luminescent Proteins - biosynthesis
Luminescent Proteins - genetics
Mammary gland diseases
Medical sciences
Receptors, Estrogen - physiology
Response Elements - genetics
Tumors
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Summary:To gain insight into the in vivo role of estrogen, we isolated estrogen receptor-positive cells fromnormal human breast tissue using a recombinant adenovirus that expresses green fluorescence protein in response to estrogen. We compared the global gene expression profile of these estrogen receptor-positive cells with that of various normal and cancerous mammary epithelial cells and identified several genes not implicated previously in estrogen signaling. One of these genes, lipocalin 2, is a putative in vivo estrogen target gene and paracrine factor that mediates the growth regulatory effects of estrogen in normal breast epithelium. These results demonstrate that normal and cancerous estrogen receptor-positive cells are distinct at the molecular level and suggest that lipocalin 2 is a new therapeutic target for breast cancer prevention and treatment.
ISSN:0008-5472
1538-7445