Determination of Salbutamol in Human Plasma and Urine by High-Performance Liquid Chromatography with a Coulometric Electrode Array System

A method is developed to determine salbutamol in human plasma and urine using high-performance liquid chromatography (HPLC) with a coulometric electrode array system, based on the electrochemical behavior of salbutamol at graphite electrode. The mobile phase component A is 30mM sodium dihydroxy phos...

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Bibliographic Details
Published in:Journal of chromatographic science 2004-05, Vol.42 (5), p.263-267
Main Authors: Zhang, X.Z., Gan, Y.R., Zhao, F.N.
Format: Article
Language:English
Subjects:
Adrenergic beta-Agonists - blood
Adrenergic beta-Agonists - urine
Albuterol - blood
Albuterol - urine
Analysis
Biological and medical sciences
Calibration
Chromatography, High Pressure Liquid - methods
Electrochemistry - instrumentation
Electrochemistry - methods
General pharmacology
Humans
Medical sciences
Pharmacology. Drug treatments
Reproducibility of Results
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Summary:A method is developed to determine salbutamol in human plasma and urine using high-performance liquid chromatography (HPLC) with a coulometric electrode array system, based on the electrochemical behavior of salbutamol at graphite electrode. The mobile phase component A is 30mM sodium dihydroxy phosphate-30mM triethylamine and is adjusted to pH 6.0 with 20% phosphate acid. The mobile phase component B is methanol. The optimized mobile phase composition was A and B in the proportion of 90:10 (v/v). Paracetamol is selected as the external standard. The human plasma and urine samples are pretreated using solid-phase extraction cartridges (Sep-Pak Silica), and the eluting solution is monitored by the coulometric electrode array system. The electrode potentials are set at 300, 400, 550, and 650 mV, respectively. Calibration curves show good linearity, and the recovery of salbutamol proves to be constant and unaffected by the concentration of the drug. This method, developed using HPLC-electrochemical detection, is reproducible and sensitive enough for the determination of salbutamol in human plasma and urine.
ISSN:0021-9665
1945-239X
DOI:10.1093/chromsci/42.5.263