Evaluation of the Antioxidant Effects of Some Flavonylthiazolidinediones by Determining Their Effects on Lipid Peroxidation, Superoxide Anion Formation, and 2,2-Diphenyl-1-picrylhydrazyl Stable Free Radical

• Published in: Biological & pharmaceutical bulletin 2004, Vol.27(6), pp.912-915
• Main Authors: Tuncbilek, Meral, Eke, Benay Can, Ayhan-Kilcigil, Gülgün, Çoban, Tülay, Iscan, Mümtaz
• Format: Article
• Language: English
• Subjects: 2,2-diphenyl-1-picrylhydrazyl (DPPH); Animals; antioxidant activity; Antioxidants - chemistry; Antioxidants - pharmacology; Biphenyl Compounds; Drug Evaluation, Preclinical - methods; Flavones - chemistry; Flavones - pharmacology; flavonylthiazolidinedione; Free Radicals - metabolism; lipid peroxidation; Lipid Peroxidation - drug effects; Male; Microsomes, Liver - drug effects; Microsomes, Liver - metabolism; Picrates - metabolism; Rats; Rats, Wistar; superoxide dismutase; Superoxides - metabolism; Thiazolidinediones - pharmacology
• ISSN: 0918-6158; 1347-5215
• DOI: 10.1248/bpb.27.912

The in vitro antioxidant effects of some flavonylthiazolidinediones (Ia—d, IIa—d) on rat liver microsomal NADPH-dependent lipid peroxidation (LP) levels were determined by measuring the formation of 2-thiobarbituric acid reactive substance. The free radical-scavenging properties of the compounds were examined in vitro by determining the capacity to scavenge superoxide anion formation and of the interaction with the stable free radical 2,2-diphenyl-1-picrylhydrazyl (DPPH). The compounds had no inhibitory effects on LP. However, they had variable inhibitory influence on superoxide anion production and DPPH radical in a concentration-dependent manner. The most active compound, 3-(2,5-dimethoxyphenacyl)-5-[2-phenyl-4H-4-oxo-1-benzopyran-6-yl)methylenyl]-thiazolidine-2,4-dione, Id showed 98% inhibition of superoxide anion production and 95% inhibition of DPPH stable free radical at 10−3 M.