MDR1 gene expression in hepatocellular carcinoma and the peritumoral liver of patients with and without cirrhosis

P-glycoprotein (P-gp) and MDR1 mRNA expressions were assessed in tumoral and peritumoral specimens from patients with hepatocellular carcinoma (HCC) and in cirrhotic livers without HCC, using immunohistochemistry (C494 monoclonal antibody) and reverse transcription-polymerase chain reaction (RT-PCR)...

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Bibliographic Details
Published in:Cancer letters 2002-12, Vol.186 (1), p.107-113
Main Authors: Grudé, Pascale, Conti, Filomena, Mennecier, Didier, Louvel, Albert, Houssin, Didier, Weill, Bernard, Calmus, Yvon
Format: Article
Language:English
Subjects:
Adult
Aged
Animal tumors. Experimental tumors
ATP Binding Cassette Transporter, Subfamily B, Member 1 - analysis
Biological and medical sciences
Carcinoma, Hepatocellular - chemistry
Carcinoma, Hepatocellular - metabolism
Chemotherapy
Cirrhosis
Experimental digestive system and abdominal tumors
Gene expression
General aspects (metabolism, cell proliferation, established cell line...)
Genes, MDR
Hepatocellular carcinoma
Humans
Immunohistochemistry
Liver
Liver - chemistry
Liver - metabolism
Liver cancer
Liver Cirrhosis - metabolism
Liver Neoplasms - chemistry
Liver Neoplasms - metabolism
MDR1
Medical sciences
Middle Aged
P-glycoprotein
Patients
Polymerase chain reaction
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger - analysis
Rodents
Tumor cell
Tumors
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Summary:P-glycoprotein (P-gp) and MDR1 mRNA expressions were assessed in tumoral and peritumoral specimens from patients with hepatocellular carcinoma (HCC) and in cirrhotic livers without HCC, using immunohistochemistry (C494 monoclonal antibody) and reverse transcription-polymerase chain reaction (RT-PCR) analysis. P-gp overexpression was detected in 24/28 tumoral livers (85%). In the peritumoral liver, staining was strong in cirrhotic nodules, and fainter in non-cirrhotic specimens. P-gp expression was as intense in the cirrhotic specimens free of HCC as in the peritumoral tissue of HCC developing in cirrhotic patients. These results were confirmed by RT-PCR analysis.
ISSN:0304-3835
1872-7980
DOI:10.1016/S0304-3835(02)00155-6