S-Adenosyl methionine/S-adenosyl-L-homocysteine ratio determination by capillary electrophoresis employed as a monitoring tool for the antiviral effectiveness of adenosine analogs

S‐Adenosyl‐L‐homocysteine hydrolase (SAHh) inhibitors have long been used as broad‐range antivirals and have been recently evaluated as an experimental therapy of filovirus infections. In response to the need for a rapid laboratory testing method that could assess antiviral potency in vivo, our grou...

Full description

Saved in:
Bibliographic Details
Published in:Electrophoresis 2004-06, Vol.25 (10-11), p.1518-1521
Main Authors: Sbrana, Elena, Bramanti, Emilia, Spinetti, Maria C., Raspi, Giorgio
Format: Article
Language:English
Subjects:
Acetaldehyde - analogs & derivatives
Acetaldehyde - chemistry
Adenosine analogs
Antiviral Agents - pharmacology
Antiviral drugs
Ebola
Electrophoresis, Capillary
Filovirus
Humans
S-Adenosyl-L-homocysteine hydrolase
S-Adenosylhomocysteine - analysis
S-Adenosylhomocysteine - blood
S-Adenosylmethionine - analysis
S-Adenosylmethionine - blood
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:S‐Adenosyl‐L‐homocysteine hydrolase (SAHh) inhibitors have long been used as broad‐range antivirals and have been recently evaluated as an experimental therapy of filovirus infections. In response to the need for a rapid laboratory testing method that could assess antiviral potency in vivo, our group developed a capillary electrophoresis (CE) method for the determination of the S‐adenosyl‐L‐homocysteine (SAH) to S‐adenosyl‐L‐methionine (SAM) ratio. After chloroacetaldehyde derivatization, SAH and SAM were detected using laser‐induced fluorescence detection with a HeCd laser. Separation and quantitation of both SAH and SAM in human plasma were achieved in less than 1 min. The proposed method is rapid and reliable, and could be easily applied to routine monitoring of clinical and preclinical trials subjects.
ISSN:0173-0835
1522-2683
DOI:10.1002/elps.200305851