Depletion of Oestrogen Receptor-β Expression in Magnocellular Arginine Vasopressin Neurones by Hypovolaemia and Dehydration

• Published in: Journal of neuroendocrinology 2004-06, Vol.16 (6), p.544-549
• Main Authors: Somponpun, S. J., Sladek, C. D.
• Format: Article
• Language: English
• Subjects: Animals; Arginine Vasopressin - metabolism; Biological and medical sciences; Dehydration - metabolism; Down-Regulation; Estrogen Receptor beta; Fos; Fundamental and applied biological sciences. Psychology; Hypovolemia - metabolism; Male; Neurons - metabolism; Paraventricular Hypothalamic Nucleus - cytology; Paraventricular Hypothalamic Nucleus - metabolism; paraventricular nucleus; polyethylene glycol; Proto-Oncogene Proteins c-fos - metabolism; Rats; Receptors, Estrogen - metabolism; supraoptic nucleus; Supraoptic Nucleus - cytology; Supraoptic Nucleus - metabolism; Time Factors; Vertebrates: endocrinology; water deprivation; Water-Electrolyte Balance - physiology
• ISSN: 0953-8194; 1365-2826
• DOI: 10.1111/j.1365-2826.2004.01200.x

Oestrogen receptor (ER)‐β expression correlates inversely with osmotic control of arginine vasopressin (AVP) release such that cellular dehydration induced by 72 h of 2% saline consumption depletes ER‐β in the magnocellular AVP neurones in the supraoptic (SON) and paraventricular nuclei (PVN). The current studies were performed to determine whether other pathways that stimulate AVP release, such as hypovolaemia, also regulate ER‐β expression in these nuclei, and to evaluate the time course of the change in ER‐β expression during water deprivation and subsequent rehydration. ER‐β expression was evaluated immunocytochemically. In rats made hypovolaemic with a subcutaneous injection of 40% polyethylene glycol (PEG), a significant depletion of ER‐β in both SON and magnocellular PVN (P ≤ 0.001) was evident 8 h post PEG injection. ER‐β was also significantly depleted following 20 h and 26 h of water deprivation in SON (P = 0.003) and magnocellular PVN (P