Identification of Puerarin and Its Metabolites in Rats by Liquid Chromatography−Tandem Mass Spectrometry

Puerarin (daidzein-8-C-glucoside) is the major bioactive isoflavone of kudzu root (the root of Pueraria lobata). Its metabolic fate, however, is not well-known. In this study, a sensitive and specific LC-ESI-MS/MS method for the determination of puerarin and its metabolites daidzein, dihydrodaidzein...

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Bibliographic Details
Published in:Journal of agricultural and food chemistry 2004-06, Vol.52 (12), p.3708-3712
Main Authors: Prasain, Jeevan K, Jones, Kenneth, Brissie, Nancy, Moore, Ray, Wyss, J. Michael, Barnes, Stephen
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Blood
blood serum
brain
Brain Chemistry
Chromatography, Liquid - methods
daidzein
dihydrodaidzein
dihydroxydaidzein
Equol
feces
Feeding. Feeding behavior
Fundamental and applied biological sciences. Psychology
heart
isoflavones
Isoflavones - analysis
Isoflavones - metabolism
Isoflavones - pharmacokinetics
liquid chromatography
Male
mass spectrometry
Mass Spectrometry - methods
medicinal plants
metabolism
metabolites
monohydroxydaidzein
Myocardium - chemistry
oral administration
pharmacokinetics
Pueraria montana var. lobata
Rats
Rats, Sprague-Dawley
Urine
Vertebrates: anatomy and physiology, studies on body, several organs or systems
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Summary:Puerarin (daidzein-8-C-glucoside) is the major bioactive isoflavone of kudzu root (the root of Pueraria lobata). Its metabolic fate, however, is not well-known. In this study, a sensitive and specific LC-ESI-MS/MS method for the determination of puerarin and its metabolites daidzein, dihydrodaidzein, and equol was developed for their analysis in biological samples. Two new metabolites of puerarin, mono- and dihydroxylated derivatives, were detected in the urine and feces of rats after oral administration. The persistence of puerarin in blood and urine as the principal metabolic form for the period of 4−72 h after oral administration suggested that puerarin is rapidly absorbed from the intestine without metabolism. Its presence in organs such as the brain suggests that this glucoside may enter tissues by specific transport pathways. Study of these metabolites may provide further understanding of the health beneficial effects of puerarin in kudzu dietary supplements. Keywords: Puerarin; metabolites; C-glucoside; kudzu; LC-MS/MS; rats
ISSN:0021-8561
1520-5118
DOI:10.1021/jf040037t