Endothelin content, expression, and receptor type in normal and diseased human gallbladder

The aims of this study were to characterize the endothelin (ET) system in human gallbladder by determining (1) the tissue content of ET-1 and ET-2 by ELISA; (2) the expression of mRNA of the ET precursors preproendothelin-1, -2, and -3; and (3) mRNA expression for the ETA and ETB receptors. Median c...

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Bibliographic Details
Published in:Digestive diseases and sciences 2002-08, Vol.47 (8), p.1786-1792
Main Authors: MCCARTNEY, S. A, GREAVES, R. R. S. H, WARNER, T. D, O'DONNELL, L. J. D, DOMIZIO, P, FARTHING, M. J. G
Format: Article
Language:English
Subjects:
Biological and medical sciences
Cholecystitis - metabolism
Digestive system
Endothelin-1 - analysis
Endothelin-1 - genetics
Endothelin-2 - analysis
Endothelin-2 - genetics
Endothelins - genetics
Enzyme-Linked Immunosorbent Assay
Gallbladder - chemistry
Humans
Investigative techniques, diagnostic techniques (general aspects)
Medical sciences
Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques
Polymerase Chain Reaction
Protein Precursors - genetics
Receptor, Endothelin A
Receptor, Endothelin B
Receptors, Endothelin - analysis
Receptors, Endothelin - genetics
RNA, Messenger - analysis
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Summary:The aims of this study were to characterize the endothelin (ET) system in human gallbladder by determining (1) the tissue content of ET-1 and ET-2 by ELISA; (2) the expression of mRNA of the ET precursors preproendothelin-1, -2, and -3; and (3) mRNA expression for the ETA and ETB receptors. Median content of ET-1/2 was significantly reduced in severely inflamed gallbladders compared to gallbladders with mild inflammation. There was an inverse correlation between content of ET-1/2 and inflammation score. mRNA for preproendothelin-2 was highly expressed in all samples, whereas mRNA for preproendothelin-1 was present in negligible quantities and mRNA for preproendothelin-3 was undetectable. mRNA for ETA receptors was expressed in all samples analyzed, whereas mRNA for ETB receptors was expressed at a much lower level. This study demonstrates the presence of ET-1/2 in human gallbladder. ET-1/2 content is decreased with increasing degrees of histological inflammation. ET-2 is likely to be the physiologically significant endothelin isopeptide expressed and ETA receptors appear to predominate in the human gallbladder.
ISSN:0163-2116
1573-2568
DOI:10.1023/A:1016532228836