High P‐glycoprotein‐mediated export observed in patients with a history of idiopathic thrombocytopenic purpura

Studies have suggested that high P‐glycoprotein expression in lymphocytes from patients with autoimmune disorders may affect disease outcome. Idiopathic thrombocytopenic purpura (ITP) and Evans' syndrome are widely thought to be autoimmune processes, however, the precise mechanisms remain unkno...

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Bibliographic Details
Published in:British journal of haematology 2002-09, Vol.118 (3), p.836-838
Main Authors: Levy, Adam S., Cunningham‐Rundles, Susanna, Mazza, BethAnne, Simm, Maciej, Gorlick, Richard, Bussel, James
Format: Article
Language:English
Subjects:
Adolescent
Adult
Aged
ATP Binding Cassette Transporter, Subfamily B, Member 1 - blood
Autoimmune Diseases - blood
Biological and medical sciences
Cell Culture Techniques
Evans' syndrome
Female
Fluorescent Dyes - pharmacokinetics
Hematologic and hematopoietic diseases
Humans
idiopathic thrombocytopenic purpura
Lymphocytes - metabolism
Male
Medical sciences
Middle Aged
multi‐drug resistance
Platelet diseases and coagulopathies
Purpura, Thrombocytopenic, Idiopathic - blood
P‐glycoprotein
Reverse Transcriptase Polymerase Chain Reaction
rhodamine 123
Rhodamine 123 - pharmacokinetics
Syndrome
Thrombocytopenia - blood
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Summary:Studies have suggested that high P‐glycoprotein expression in lymphocytes from patients with autoimmune disorders may affect disease outcome. Idiopathic thrombocytopenic purpura (ITP) and Evans' syndrome are widely thought to be autoimmune processes, however, the precise mechanisms remain unknown. Peripheral blood mononuclear cells from patients with refractory or recurrent ITP or Evans' syndrome were studied using the rhodamine 123 flow cytometric assay to investigate functional export levels. Lymphocytes from ITP and Evans' syndrome patients showed a significantly decreased ability to retain rhodamine, suggesting increased export protein function. Reverse transcription polymerase chain reaction distinguished P‐glycoprotein as the likely export protein.
ISSN:0007-1048
1365-2141
DOI:10.1046/j.1365-2141.2002.03709.x