High overall diversity and dominance of microdiverse relationships in salt marsh sulphate-reducing bacteria

Summary The biogeochemistry of North Atlantic salt marshes is characterized by the interplay between the marsh grass Spartina and sulphate‐reducing bacteria (SRB), which mineralize the diverse carbon substrates provided by the plants. It was hypothesized that SRB populations display high diversity w...

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Bibliographic Details
Published in:Environmental microbiology 2004-07, Vol.6 (7), p.686-698
Main Authors: Klepac-Ceraj, Vanja, Bahr, Michele, Crump, Byron C., Teske, Andreas P., Hobbie, John E., Polz, Martin F.
Format: Article
Language:English
Subjects:
Atlantic Islands
Biodiversity
Deltaproteobacteria - classification
Deltaproteobacteria - genetics
Deltaproteobacteria - isolation & purification
Deltaproteobacteria - metabolism
Desulfobacterium
Desulfococcus
Desulfosarcina
DNA, Bacterial - chemistry
DNA, Bacterial - isolation & purification
DNA, Ribosomal - chemistry
DNA, Ribosomal - isolation & purification
Environmental Microbiology
Genes, rRNA - genetics
Geologic Sediments - microbiology
Molecular Sequence Data
Oxidation-Reduction
Phylogeny
Poaceae - growth & development
Poaceae - metabolism
Poaceae - microbiology
RNA, Ribosomal, 16S - genetics
Sequence Analysis, DNA
Sequence Homology, Nucleic Acid
Spartina
Sulfates - metabolism
Sulfur-Reducing Bacteria - classification
Sulfur-Reducing Bacteria - genetics
Sulfur-Reducing Bacteria - isolation & purification
Sulfur-Reducing Bacteria - metabolism
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Summary:Summary The biogeochemistry of North Atlantic salt marshes is characterized by the interplay between the marsh grass Spartina and sulphate‐reducing bacteria (SRB), which mineralize the diverse carbon substrates provided by the plants. It was hypothesized that SRB populations display high diversity within the sediment as a result of the rich spatial and chemical structuring provided by Spartina roots. A 2000‐member 16S rRNA gene library, prepared with delta‐proteobacterial SRB‐selective primers, was analysed for diversity patterns and phylogenetic relationships. Sequence clustering detected 348 16S rRNA sequence types (ribotypes) related to delta‐proteobacterial SRB, and it was estimated that a total of 623 ribotypes were present in the library. Similarity clustering showed that ≈ 46% of these sequences fell into groups with
ISSN:1462-2912
1462-2920
DOI:10.1111/j.1462-2920.2004.00600.x