Proteomics analysis of human endothelial cell line EA.hy926 after exposure to GSM 900 radiation

The human endothelial cell line EA.hy926 was exposed to mobile phone radiation and the effect on protein expression was examined using two‐dimensional electrophoresis (2‐DE). Up to 38 various proteins have statistically significantly altered their expression levels following the irradiation. Four pr...

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Bibliographic Details
Published in:Proteomics (Weinheim) 2004-05, Vol.4 (5), p.1359-1365
Main Authors: Nylund, Reetta, Leszczynski, Dariusz
Format: Article
Language:English
Subjects:
Actins - metabolism
Cell Line
Cell Phone
Electrophoresis, Gel, Two-Dimensional
Electrophoresis, Polyacrylamide Gel
Endothelium
Endothelium, Vascular - chemistry
Endothelium, Vascular - growth & development
Endothelium, Vascular - radiation effects
Heat-Shock Proteins - metabolism
Human endothelial cell line
Humans
Isoelectric Focusing
Mass Spectrometry
Microwaves
Microwaves - adverse effects
Mobile phone radiation
Protein Isoforms - chemistry
Protein Isoforms - genetics
Proteome - analysis
Proteomics - methods
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Vimentin - chemistry
Vimentin - genetics
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Summary:The human endothelial cell line EA.hy926 was exposed to mobile phone radiation and the effect on protein expression was examined using two‐dimensional electrophoresis (2‐DE). Up to 38 various proteins have statistically significantly altered their expression levels following the irradiation. Four proteins were identified with matrix‐assisted laser desorption/ionization‐mass spectrometry (MALDI‐MS). Two of the affected proteins were determined to be isoforms of cytoskeletal vimentin. This finding supports our earlier presented working hypothesis which indicated that the mobile phone radiation might affect the cytoskeleton and might have an effect on the physiological functions that are regulated by the cytoskeleton.
ISSN:1615-9853
1615-9861
DOI:10.1002/pmic.200300773