Molecular signature linked to acquired resistance to cisplatin in esophageal cancer cells

• Published in: Cancer letters 2004-07, Vol.211 (1), p.69-78
• Main Authors: Toshimitsu, Hiroaki, Hashimoto, Kiichiro, Tangoku, Akira, Iizuka, Norio, Yamamoto, Kohtaro, Kawauchi, Shigeto, oga, Atsunori, Furuya, Tomoko, Oka, Masaaki, Sasaki, Kohsuke
• Format: Article
• Language: English
• Subjects: Acquired CDDP-resistant cell line; Antineoplastic Agents; Carcinoma, Squamous Cell - drug therapy; cDNA microarray; Cell cycle; Cell Cycle - drug effects; Cell Line, Tumor; Chromosome Aberrations - drug effects; Chromosomes; Cisplatin - pharmacology; Cisplatin - therapeutic use; Comparative genomic hybridization; Deoxyribonucleic acid; DNA; DNA Fragmentation - drug effects; Drug Resistance, Neoplasm; Esophageal Neoplasms - drug therapy; Gene Dosage; Gene Expression - drug effects; Genes; Humans; Hybridization; Kinases; Oligonucleotide Array Sequence Analysis; Polypeptides; Proteins; Reverse Transcriptase Polymerase Chain Reaction; Ribosomal DNA; Ribosomal protein
• ISSN: 0304-3835; 1872-7980
• DOI: 10.1016/j.canlet.2004.01.038

To clarify the molecular basis of acquired cisplatin (CDDP) resistance in esophageal squamous cell carcinoma (ESCC), we used cDNA microarray technology. A CDDP-resistant cell line (YES-2/CDDP), which shows a 7.5-fold increase in resistance to CDDP and a 3-fold decrease in CDDP accumulation compared with the parental YES-2 ESCC cell line, was generated from YES-2 by exposure to increased concentrations of CDDP. By cDNA microarray analysis, we identified 44 genes with significantly different expression levels between YES-2/CDDP and YES-2 cells. Interestingly, 15 of these 44 genes encoded ribosome-related proteins, almost all of which were underexpressed in YES-2/CDDP cells. Our present data suggest that many ribosome-related genes may be involved in the acquired resistance to CDDP in ESCC and that such information may allow us to better understand the mechanism of CDDP resistance.