Interaction of oxoferrylmyoglobin and dinitrosyl-iron complexes

It is shown that dinitrosyl-iron complexes (DNIC) with glutathione can reduce oxoferrylmyoglobin forming on interaction of tert-butyl hydroperoxide and metmyoglobin. A rapid decrease in the DNIC concentration was observed under the conditions of production of tert-butyl free radicals; however, destr...

Full description

Saved in:
Bibliographic Details
Published in:Biochemistry (Moscow) 2004-05, Vol.69 (5), p.569-574
Main Authors: Shumaev, K B, Petrova, N E, Zabbarova, I V, Vanin, A F, Topunov, A F, Lankin, V Z, Ruuge, E K
Format: Article
Language:English
Subjects:
Antioxidants - metabolism
Cyclic N-Oxides - metabolism
Electron Spin Resonance Spectroscopy
Free radicals
Free Radicals - metabolism
Glutathione - metabolism
Iron - metabolism
Metmyoglobin - metabolism
Nitric Oxide
Nitrogen Oxides - metabolism
Peroxides
S-Nitrosoglutathione - metabolism
tert-Butylhydroperoxide - metabolism
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:It is shown that dinitrosyl-iron complexes (DNIC) with glutathione can reduce oxoferrylmyoglobin forming on interaction of tert-butyl hydroperoxide and metmyoglobin. A rapid decrease in the DNIC concentration was observed under the conditions of production of tert-butyl free radicals; however, destruction of DNIC in the presence of oxoferrylmyoglobin alone was negligible. It is demonstrated that DNIC reduces oxoferrylmyoglobin more than an order more efficiently than S-nitrosoglutathione and glutathione. DNIC also inhibits formation of the thiyl radicals of glutathione in a medium containing metmyoglobin and tert-butyl hydroperoxide. A mechanism of the antioxidant action of DNIC based on regeneration of the nitrosyl complexes from the products of their interaction with oxoferrylheme is proposed.
ISSN:0006-2979
1608-3040
DOI:10.1023/B:BIRY.0000029856.67884.c5