Selective alterations in insulin receptor substrates-1, -2 and -4 in theca but not granulosa cells from polycystic ovaries

• Published in: Molecular human reproduction 2004-07, Vol.10 (7), p.473-479
• Main Authors: Yen, H-W., Jakimiuk, A.J., Munir, I., Magoffin, D.A.
• Format: Article
• Language: English
• Subjects: Adaptor Proteins, Signal Transducing; Adult; Animals; Biological and medical sciences; Embryology: invertebrates and vertebrates. Teratology; Female; Fundamental and applied biological sciences. Psychology; granulosa cell; Granulosa Cells - cytology; Granulosa Cells - metabolism; Humans; Insulin - metabolism; insulin receptor substrate; Insulin Receptor Substrate Proteins; insulin signalling; Intracellular Signaling Peptides and Proteins; PCOS; Phosphoproteins - metabolism; Polycystic Ovary Syndrome - metabolism; Receptor, Insulin - metabolism; Signal Transduction - physiology; theca cell; Theca Cells - cytology; Theca Cells - metabolism
• ISSN: 1360-9947; 1460-2407; 1460-2407
• DOI: 10.1093/molehr/gah066

The elevated insulin concentrations that occur in many women with polycystic ovary syndrome (PCOS) can contribute significantly to ovarian hyperandrogenism. The objective of the present study was to compare the content of proximal insulin signalling molecules in theca and granulosa cells between polycystic ovaries and regular cycling controls. Individual follicles \batchmode \documentclass[fleqn,10pt,legalpaper]{article} \usepackage{amssymb} \usepackage{amsfonts} \usepackage{amsmath} \pagestyle{empty} \begin{document} \((3{\mbox{--}}7{\,}mm)\) \end{document} were obtained from 11 women with PCOS and 10 regularly cycling control women. The theca and granulosa cells were microdissected from each follicle. Total protein was extracted and signalling proteins were measured by western blot analysis. There was no difference in insulin receptor content between PCOS and controls in either theca or granulosa cells. Insulin receptor substrate (IRS)-1 and -2 were increased \batchmode \documentclass[fleqn,10pt,legalpaper]{article} \usepackage{amssymb} \usepackage{amsfonts} \usepackage{amsmath} \pagestyle{empty} \begin{document} \((\mathit{P}{