L-arginine infusion decreases plasma total homocysteine concentrations through increased nitric oxide production and decreased oxidative status in Type II diabetic patients

Hyperhomocysteinaemia increases cardiovascular risk in Type II (non-insulin-dependent) diabetes mellitus by augmenting oxidative stress and reducing nitric oxide availability. In vitro, nitric oxide decreases homocysteine by its conversion to the vasodilative and antioxidant compound S-nitrosohomocy...

Full description

Saved in:
Bibliographic Details
Published in:Diabetologia 2002-08, Vol.45 (8), p.1120-1127
Main Authors: FALDETTA, M. Cassone, LAURENTI, O, DESIDERI, G, BRAVI, M. C, DE LUCA, O, MARINUCCI, M. C, DE MATTIA, G, FERRI, C
Format: Article
Language:English
Subjects:
Arginine - administration & dosage
Arginine - pharmacology
Associated diseases and complications
Biological and medical sciences
Blood Pressure - drug effects
Cross-Over Studies
Diabetes Mellitus, Type 2 - metabolism
Diabetes. Impaired glucose tolerance
Double-Blind Method
Endocrine pancreas. Apud cells (diseases)
Endocrinopathies
Female
Homocysteine - blood
Humans
Infusions, Intravenous
Male
Medical sciences
Middle Aged
Nitrates - blood
Nitric Oxide - biosynthesis
Nitrites - blood
Osmolar Concentration
Oxidative Stress
Reference Values
Sulfhydryl Compounds - blood
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Hyperhomocysteinaemia increases cardiovascular risk in Type II (non-insulin-dependent) diabetes mellitus by augmenting oxidative stress and reducing nitric oxide availability. In vitro, nitric oxide decreases homocysteine by its conversion to the vasodilative and antioxidant compound S-nitrosohomocysteine. We investigated whether or not changes in nitric oxide availability decrease homocysteine concentrations in vivo. The study group consisted of 20 normotensive, normolipidaemic, non-atherosclerotic Type II diabetic patients in good metabolic control (16 men, 51.2+/-1.4 years) and 15 healthy subjects (12 men, 48.1+/-1.5 years). Circulating concentrations of homocysteine, nitrite+nitrate and sulphydryl groups, a marker of oxidative stress, were assessed at baseline and then 5', 10', 30' and 60' after the intravenous infusion of either L-arginine (3 g in 10 ml saline), the nitric oxide precursor, or vehicle according to a double-blind cross-over randomized protocol. At baseline diabetic patients showed lower plasma sulphydryl group concentrations (491.8+/-16.9 vs 551.3+/-21.0 micro mol/l, p
ISSN:0012-186X
1432-0428
DOI:10.1007/s00125-002-0854-1