Cytokine-induced upregulation of NF-kappaB, IL-8, and ICAM-1 is dependent on colonic cell polarity: implication for PKCdelta

• Published in: Experimental cell research 2004-07, Vol.297 (1), p.165-185
• Main Authors: Vallee, Sebastien, Laforest, Sullivan, Fouchier, Francis, Montero, Marie Pierre, Penel, Claude, Champion, Serge
• Format: Article
• Language: English
• Subjects: Adenocarcinoma - enzymology; Adenocarcinoma - genetics; Adenocarcinoma - immunology; Cell Differentiation - drug effects; Cell Differentiation - genetics; Cell Line, Tumor; Cell Membrane - drug effects; Cell Membrane - enzymology; Cell Membrane - genetics; Cell Polarity - drug effects; Cell Polarity - physiology; Colonic Neoplasms - enzymology; Colonic Neoplasms - genetics; Colonic Neoplasms - immunology; Cytokines - metabolism; Cytokines - pharmacology; Drug Tolerance - genetics; Epithelial Cells - cytology; Epithelial Cells - immunology; Epithelial Cells - metabolism; Gene Expression Regulation, Enzymologic - drug effects; Gene Expression Regulation, Enzymologic - genetics; Humans; Intercellular Adhesion Molecule-1 - genetics; Intercellular Adhesion Molecule-1 - metabolism; Interleukin-1 - metabolism; Interleukin-1 - pharmacology; Interleukin-8 - genetics; Interleukin-8 - metabolism; Intestinal Mucosa - cytology; Intestinal Mucosa - immunology; Intestinal Mucosa - metabolism; Microvilli - drug effects; Microvilli - enzymology; Microvilli - genetics; NF-kappa B - genetics; NF-kappa B - metabolism; Protein Kinase C - metabolism; Protein Kinase C-delta; Protein Transport - drug effects; Protein Transport - genetics; Transcriptional Activation; Tumor Necrosis Factor-alpha - metabolism; Tumor Necrosis Factor-alpha - pharmacology; Up-Regulation - drug effects; Up-Regulation - genetics
• ISSN: 0014-4827

As described for a long time, carcinoma-derived Caco-2 cells form a polarized epithelium in culture, whereas HT29-D4 cells are nonpolarized and undifferentiated but can form a polarized monolayer when cultured in a galactose-supplemented medium. Using NF-kappaB translocation and IL-8 and ICAM-1 gene activation as an index, we have studied the relationship between the differentiation state and the cell response to cytokines. We found that differentiated Caco-2 and HT29-D4 cells were responsive to both cytokines TNFalpha- and IL-1beta-mediated activation of NF-kappaB but that undifferentiated HT29-D4 cells were unresponsive to IL-1beta. However, the expression of endogenous ICAM-1 and IL-8 genes was upregulated by these cytokines in either cell lines differentiated or not. Upregulation of ICAM-1 gene occurred when IL-1beta or TNFalpha was added to the basal, but not apical surface of the differentiated epithelia. Finally, it appeared that in polarized HT29-D4 cells, the IL-1beta-induced translocation of NF-kappaB was connected to PKCdelta translocation.