Loading…
Pyruvate formation and suppression in recombinant Bacillus megaterium cultivation
A recombinant Bacillus megaterium strain showed the ability to secrete large amounts of pyruvate (up to 27.8 g l −1) for growth rates larger than 0.15 h −1. Cultivation below this growth rate avoids pyruvate formation while minimizing acetate and succinate production. Using exponential feeding, fina...
Saved in:
| Published in: | Journal of biotechnology 2004-07, Vol.111 (1), p.89-96 |
|---|---|
| Main Authors: | , |
| Format: | Article |
| Language: | English |
| Subjects: | |
| Citations: | Items that this one cites Items that cite this one |
| Online Access: | Get full text |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| cited_by | cdi_FETCH-LOGICAL-c525t-18c453f68ae3824ed8b6bc764ced680078c02ea2564c0185f188522c67acd49d3 |
|---|---|
| cites | cdi_FETCH-LOGICAL-c525t-18c453f68ae3824ed8b6bc764ced680078c02ea2564c0185f188522c67acd49d3 |
| container_end_page | 96 |
| container_issue | 1 |
| container_start_page | 89 |
| container_title | Journal of biotechnology |
| container_volume | 111 |
| creator | Hollmann, Rajan Deckwer, Wolf-Dieter |
| description | A recombinant
Bacillus megaterium strain showed the ability to secrete large amounts of pyruvate (up to 27.8
g
l
−1) for growth rates larger than 0.15
h
−1. Cultivation below this growth rate avoids pyruvate formation while minimizing acetate and succinate production. Using exponential feeding, final biomass concentrations of up to 80
g
l
−1 were achieved. Overall molar yields for the experiments with pyruvate formation were as high as 0.79
mol
mol
−1. Pyruvate formation was caused by the discrepancy between glycolytic and pyruvate dehydrogenase reaction/tricarboxylic acid cycle capacities during glucose excess. High pyruvate resulted in deceleration and subsequent cessation of growth. In addition, this inhibitory effect is likely associated with the phoshoenolpyruvate:glucose phosphotransferase system used by
B. megaterium as the main importer for glucose. |
| doi_str_mv | 10.1016/j.jbiotec.2004.03.006 |
| format | article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_72022679</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0168165604001610</els_id><sourcerecordid>72022679</sourcerecordid><originalsourceid>FETCH-LOGICAL-c525t-18c453f68ae3824ed8b6bc764ced680078c02ea2564c0185f188522c67acd49d3</originalsourceid><addsrcrecordid>eNqFkctKxDAUhoMozjj6CEo3umtN0ubSlejgDQZU0HVI01NJ6WVM2oF5ezNOQXeuDjl8fxK-H6FzghOCCb-uk7qw_QAmoRhnCU4TjPkBmhMp0jiTPD1E88DJmHDGZ-jE-xoHMGfkGM0IIzkXIp2jt9etGzd6gKjqXasH23eR7srIj-u1A-93Z9tFDkzfFrbT3RDdaWObZvRRC58h6OzYRmZsBrv5iZ-io0o3Hs6muUAfD_fvy6d49fL4vLxdxYZRNsREmoylFZcaUkkzKGXBCyN4ZqDkEmMhDaagKQsbTCSriJSMUsOFNmWWl-kCXe3vXbv-awQ_qNZ6A02jO-hHrwTFlHKR_wsSkecsFzyAbA8a13vvoFJrZ1vttopgtZOuajVJVzvpCqcqSA-5i-mBsWih_E1NlgNwOQHaG91UTnfG-j-c5IKG3hboZs9B8Lax4JQ3FrpgxIYCBlX29p-vfAME_qN3</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>17995976</pqid></control><display><type>article</type><title>Pyruvate formation and suppression in recombinant Bacillus megaterium cultivation</title><source>ScienceDirect Journals</source><creator>Hollmann, Rajan ; Deckwer, Wolf-Dieter</creator><creatorcontrib>Hollmann, Rajan ; Deckwer, Wolf-Dieter</creatorcontrib><description>A recombinant
Bacillus megaterium strain showed the ability to secrete large amounts of pyruvate (up to 27.8
g
l
−1) for growth rates larger than 0.15
h
−1. Cultivation below this growth rate avoids pyruvate formation while minimizing acetate and succinate production. Using exponential feeding, final biomass concentrations of up to 80
g
l
−1 were achieved. Overall molar yields for the experiments with pyruvate formation were as high as 0.79
mol
mol
−1. Pyruvate formation was caused by the discrepancy between glycolytic and pyruvate dehydrogenase reaction/tricarboxylic acid cycle capacities during glucose excess. High pyruvate resulted in deceleration and subsequent cessation of growth. In addition, this inhibitory effect is likely associated with the phoshoenolpyruvate:glucose phosphotransferase system used by
B. megaterium as the main importer for glucose.</description><identifier>ISSN: 0168-1656</identifier><identifier>EISSN: 1873-4863</identifier><identifier>DOI: 10.1016/j.jbiotec.2004.03.006</identifier><identifier>PMID: 15196773</identifier><identifier>CODEN: JBITD4</identifier><language>eng</language><publisher>Lausanne: Elsevier B.V</publisher><subject>B. megaterium ; Bacillus megaterium ; Bacillus megaterium - classification ; Bacillus megaterium - genetics ; Bacillus megaterium - growth & development ; Bacillus megaterium - metabolism ; Biological and medical sciences ; Bioreactors - microbiology ; Biotechnology ; Cell Culture Techniques - methods ; Fundamental and applied biological sciences. Psychology ; Genetic Enhancement - methods ; Glucosyltransferases - genetics ; Glucosyltransferases - metabolism ; High cell density cultivation ; Pyruvate ; Pyruvic Acid - metabolism ; Recombination, Genetic - genetics ; Species Specificity ; Sucrose - metabolism</subject><ispartof>Journal of biotechnology, 2004-07, Vol.111 (1), p.89-96</ispartof><rights>2004 Elsevier B.V.</rights><rights>2004 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c525t-18c453f68ae3824ed8b6bc764ced680078c02ea2564c0185f188522c67acd49d3</citedby><cites>FETCH-LOGICAL-c525t-18c453f68ae3824ed8b6bc764ced680078c02ea2564c0185f188522c67acd49d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15867287$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15196773$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hollmann, Rajan</creatorcontrib><creatorcontrib>Deckwer, Wolf-Dieter</creatorcontrib><title>Pyruvate formation and suppression in recombinant Bacillus megaterium cultivation</title><title>Journal of biotechnology</title><addtitle>J Biotechnol</addtitle><description>A recombinant
Bacillus megaterium strain showed the ability to secrete large amounts of pyruvate (up to 27.8
g
l
−1) for growth rates larger than 0.15
h
−1. Cultivation below this growth rate avoids pyruvate formation while minimizing acetate and succinate production. Using exponential feeding, final biomass concentrations of up to 80
g
l
−1 were achieved. Overall molar yields for the experiments with pyruvate formation were as high as 0.79
mol
mol
−1. Pyruvate formation was caused by the discrepancy between glycolytic and pyruvate dehydrogenase reaction/tricarboxylic acid cycle capacities during glucose excess. High pyruvate resulted in deceleration and subsequent cessation of growth. In addition, this inhibitory effect is likely associated with the phoshoenolpyruvate:glucose phosphotransferase system used by
B. megaterium as the main importer for glucose.</description><subject>B. megaterium</subject><subject>Bacillus megaterium</subject><subject>Bacillus megaterium - classification</subject><subject>Bacillus megaterium - genetics</subject><subject>Bacillus megaterium - growth & development</subject><subject>Bacillus megaterium - metabolism</subject><subject>Biological and medical sciences</subject><subject>Bioreactors - microbiology</subject><subject>Biotechnology</subject><subject>Cell Culture Techniques - methods</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genetic Enhancement - methods</subject><subject>Glucosyltransferases - genetics</subject><subject>Glucosyltransferases - metabolism</subject><subject>High cell density cultivation</subject><subject>Pyruvate</subject><subject>Pyruvic Acid - metabolism</subject><subject>Recombination, Genetic - genetics</subject><subject>Species Specificity</subject><subject>Sucrose - metabolism</subject><issn>0168-1656</issn><issn>1873-4863</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><recordid>eNqFkctKxDAUhoMozjj6CEo3umtN0ubSlejgDQZU0HVI01NJ6WVM2oF5ezNOQXeuDjl8fxK-H6FzghOCCb-uk7qw_QAmoRhnCU4TjPkBmhMp0jiTPD1E88DJmHDGZ-jE-xoHMGfkGM0IIzkXIp2jt9etGzd6gKjqXasH23eR7srIj-u1A-93Z9tFDkzfFrbT3RDdaWObZvRRC58h6OzYRmZsBrv5iZ-io0o3Hs6muUAfD_fvy6d49fL4vLxdxYZRNsREmoylFZcaUkkzKGXBCyN4ZqDkEmMhDaagKQsbTCSriJSMUsOFNmWWl-kCXe3vXbv-awQ_qNZ6A02jO-hHrwTFlHKR_wsSkecsFzyAbA8a13vvoFJrZ1vttopgtZOuajVJVzvpCqcqSA-5i-mBsWih_E1NlgNwOQHaG91UTnfG-j-c5IKG3hboZs9B8Lax4JQ3FrpgxIYCBlX29p-vfAME_qN3</recordid><startdate>20040701</startdate><enddate>20040701</enddate><creator>Hollmann, Rajan</creator><creator>Deckwer, Wolf-Dieter</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20040701</creationdate><title>Pyruvate formation and suppression in recombinant Bacillus megaterium cultivation</title><author>Hollmann, Rajan ; Deckwer, Wolf-Dieter</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c525t-18c453f68ae3824ed8b6bc764ced680078c02ea2564c0185f188522c67acd49d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>B. megaterium</topic><topic>Bacillus megaterium</topic><topic>Bacillus megaterium - classification</topic><topic>Bacillus megaterium - genetics</topic><topic>Bacillus megaterium - growth & development</topic><topic>Bacillus megaterium - metabolism</topic><topic>Biological and medical sciences</topic><topic>Bioreactors - microbiology</topic><topic>Biotechnology</topic><topic>Cell Culture Techniques - methods</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genetic Enhancement - methods</topic><topic>Glucosyltransferases - genetics</topic><topic>Glucosyltransferases - metabolism</topic><topic>High cell density cultivation</topic><topic>Pyruvate</topic><topic>Pyruvic Acid - metabolism</topic><topic>Recombination, Genetic - genetics</topic><topic>Species Specificity</topic><topic>Sucrose - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hollmann, Rajan</creatorcontrib><creatorcontrib>Deckwer, Wolf-Dieter</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of biotechnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hollmann, Rajan</au><au>Deckwer, Wolf-Dieter</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Pyruvate formation and suppression in recombinant Bacillus megaterium cultivation</atitle><jtitle>Journal of biotechnology</jtitle><addtitle>J Biotechnol</addtitle><date>2004-07-01</date><risdate>2004</risdate><volume>111</volume><issue>1</issue><spage>89</spage><epage>96</epage><pages>89-96</pages><issn>0168-1656</issn><eissn>1873-4863</eissn><coden>JBITD4</coden><abstract>A recombinant
Bacillus megaterium strain showed the ability to secrete large amounts of pyruvate (up to 27.8
g
l
−1) for growth rates larger than 0.15
h
−1. Cultivation below this growth rate avoids pyruvate formation while minimizing acetate and succinate production. Using exponential feeding, final biomass concentrations of up to 80
g
l
−1 were achieved. Overall molar yields for the experiments with pyruvate formation were as high as 0.79
mol
mol
−1. Pyruvate formation was caused by the discrepancy between glycolytic and pyruvate dehydrogenase reaction/tricarboxylic acid cycle capacities during glucose excess. High pyruvate resulted in deceleration and subsequent cessation of growth. In addition, this inhibitory effect is likely associated with the phoshoenolpyruvate:glucose phosphotransferase system used by
B. megaterium as the main importer for glucose.</abstract><cop>Lausanne</cop><cop>Amsterdam</cop><cop>New York, NY</cop><pub>Elsevier B.V</pub><pmid>15196773</pmid><doi>10.1016/j.jbiotec.2004.03.006</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0168-1656 |
| ispartof | Journal of biotechnology, 2004-07, Vol.111 (1), p.89-96 |
| issn | 0168-1656 1873-4863 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_72022679 |
| source | ScienceDirect Journals |
| subjects | B. megaterium Bacillus megaterium Bacillus megaterium - classification Bacillus megaterium - genetics Bacillus megaterium - growth & development Bacillus megaterium - metabolism Biological and medical sciences Bioreactors - microbiology Biotechnology Cell Culture Techniques - methods Fundamental and applied biological sciences. Psychology Genetic Enhancement - methods Glucosyltransferases - genetics Glucosyltransferases - metabolism High cell density cultivation Pyruvate Pyruvic Acid - metabolism Recombination, Genetic - genetics Species Specificity Sucrose - metabolism |
| title | Pyruvate formation and suppression in recombinant Bacillus megaterium cultivation |
| url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-20T23%3A21%3A57IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Pyruvate%20formation%20and%20suppression%20in%20recombinant%20Bacillus%20megaterium%20cultivation&rft.jtitle=Journal%20of%20biotechnology&rft.au=Hollmann,%20Rajan&rft.date=2004-07-01&rft.volume=111&rft.issue=1&rft.spage=89&rft.epage=96&rft.pages=89-96&rft.issn=0168-1656&rft.eissn=1873-4863&rft.coden=JBITD4&rft_id=info:doi/10.1016/j.jbiotec.2004.03.006&rft_dat=%3Cproquest_cross%3E72022679%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c525t-18c453f68ae3824ed8b6bc764ced680078c02ea2564c0185f188522c67acd49d3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=17995976&rft_id=info:pmid/15196773&rfr_iscdi=true |