Skin reactivity to histamine and expression of histamine receptors mRNA in lymphocytes of healthy subjects and non-small-cell lung cancer patients before and after surgery

• Published in: Lung cancer (Amsterdam, Netherlands) Netherlands), 2004-07, Vol.45 (1), p.31-38
• Main Authors: Zak-Nejmark, Teresa, Jankowska, Renata, Passowicz-Muszynska, Ewa, Malolepszy, Jozef, Marciniak, Marek, Jonkisz, Anna, Kraus-Filarska, Maria
• Format: Article
• Language: English
• Subjects: Aged; Biological and medical sciences; Carcinoma, Non-Small-Cell Lung - immunology; Carcinoma, Non-Small-Cell Lung - surgery; Expression of histamine receptors mRNA; Female; Gene Expression Profiling; Histamine; Histamine receptors; Humans; Immunocompromised Host; Lung Neoplasms - immunology; Lung Neoplasms - surgery; Lymphocytes - immunology; Male; Medical sciences; Middle Aged; Non-small-cell lung cancer; Pneumology; Receptors, Histamine - biosynthesis; Receptors, Histamine - immunology; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger - biosynthesis; Skin Tests; Skin-prick test; Tumors of the respiratory system and mediastinum
• ISSN: 0169-5002; 1872-8332
• DOI: 10.1016/j.lungcan.2004.01.005

Histamine modulates an immunological response through stimulation of appropriate receptor—H1R proinflammatory or H2R suppressive The participation of histamine in regulation of an immunological response in the course of neoplastic disease is determined by the expression of particular receptor. The aim of our work was the investigation of the expression of mRNA of two types of histamine receptors in peripheral blood lymphocytes and the evaluation of skin-prick test with histamine in lung cancer patients before and after surgery. The investigation was performed on 15 patients qualified to surgery before and 7–10 days after treatment and on 12 healthy subjects. Reverse transcriptase polymerase chain reaction (RT-PCR) with primers labeled with fluorescent dyes was performed. Intensity of fluorescence was expressed as relative fluorescence units (RFU). The data were analysed using ABI Prism 310 GeneScan collection software Version 3.1. Skin-prick test with histamine was evaluated after 10 min by measuring the diameter of the weal. The expression of H1R and H2R mRNA in healthy subjects was not significantly different in contrast to the lung cancer patients in which a significant prevalence of H2R mRNA expression was observed before surgery and only slightly decreased after ( P