In vivo gene transfection via intravitreal injection of cationic liposome/plasmid DNA complexes in rabbits

To optimize the in vivo ocular transfection efficiency of plasmid DNA (pDNA)/cationic liposome complexes, N-[1-(2,3-dioleyloxy)propyl]- N, N, N-trimethylammonium chloride (DOTMA)/dioleoylphosphatidylethanolamine (DOPE) (1:1 molar ratio) liposomes and DOTMA/cholesterol (Chol) (1:1 molar ratio) liposo...

Full description

Saved in:
Bibliographic Details
Published in:International journal of pharmaceutics 2004-07, Vol.278 (2), p.255-262
Main Authors: Kawakami, Shigeru, Harada, Ayaka, Sakanaka, Koji, Nishida, Koyo, Nakamura, Junzo, Sakaeda, Toshiyuki, Ichikawa, Nobuhiro, Nakashima, Mikiro, Sasaki, Hitoshi
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Cationic liposomes
Cations
Cholesterol - chemistry
DNA - administration & dosage
DNA - chemistry
Eye
General pharmacology
Liposomes
Luciferases - biosynthesis
Luciferases - genetics
Male
Medical sciences
Pharmaceutical technology. Pharmaceutical industry
Pharmacology. Drug treatments
Phosphatidylethanolamines - chemistry
Plasmid DNA
Plasmids
Quaternary Ammonium Compounds - chemistry
Rabbits
Transfection
Transfection - methods
Vitreous body
Vitreous Body - metabolism
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:To optimize the in vivo ocular transfection efficiency of plasmid DNA (pDNA)/cationic liposome complexes, N-[1-(2,3-dioleyloxy)propyl]- N, N, N-trimethylammonium chloride (DOTMA)/dioleoylphosphatidylethanolamine (DOPE) (1:1 molar ratio) liposomes and DOTMA/cholesterol (Chol) (1:1 molar ratio) liposomes were prepared with varying amounts of pDNA. pDNA/cationic liposome complexes were intravitreally injected (100 μL) in rabbits, and luciferase activity in the cornea, aqueous humor, iris–ciliary body, lens, vitreous body, and retina was measured. Transfection efficiency of pDNA alone did not change with pDNA ranging from 40 to 85 mg. In contrast, transfection efficiency of pDNA complexed with DOTMA/Chol liposomes significantly increased with the amount of pDNA ranging from 40 to 85 μg ( P
ISSN:0378-5173
1873-3476
DOI:10.1016/j.ijpharm.2004.03.013